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Literature summary extracted from

  • Coello, P.
    Purification and characterization of secreted acid phosphatase in phosphorus-deficient Arabidopsis thaliana (2002), Physiol. Plant., 116, 293-298.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 F- 10 mM, 91% inhibition Arabidopsis thaliana
3.1.3.2 molybdate 10 mM, 98% inhibition Arabidopsis thaliana
3.1.3.2 additional information not inhibited by tartrate Arabidopsis thaliana
3.1.3.2 nitrate 10 mM, 9% inhibition Arabidopsis thaliana
3.1.3.2 phosphate 10 mM, 66% inhibition Arabidopsis thaliana
3.1.3.2 vanadate 10 mM, 96% inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.124
-
 diphosphate pH 4.8, 37°C Arabidopsis thaliana
3.1.3.2 0.378
-
 Polyphosphate pH 4.8, 37°C Arabidopsis thaliana
3.1.3.2 2.32
-
 4-nitrophenyl phosphate pH 4.8, 37°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Ca2+ 10 mM, stimulation to 142% of control Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.2 additional information Arabidopsis thaliana roots respond to the absence of an exogenous phosphate source with an increase in the specific activities of secreted acid phosphatases. The enzyme may have a role in mobilizing organic phosphate in the soil ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Arabidopsis thaliana 4-nitrophenol + phosphate
-
 ?
3.1.3.2 ADP + H2O 58% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana ?
-
 ?
3.1.3.2 ATP + H2O 68% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana ?
-
 ?
3.1.3.2 D-fructose 6-phosphate + H2O 17% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana D-fructose + phosphate
-
 ?
3.1.3.2 D-glucose 1-phosphate + H2O 8.3% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana D-glucose + phosphate
-
 ?
3.1.3.2 diphosphate + H2O 169% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana 2 phosphate
-
 ?
3.1.3.2 additional information roots respond to the absence of an exogenous phosphate source with an increase in the specific activities of secreted acid phosphatases. The enzyme may have a role in mobilizing organic phosphate in the soil Arabidopsis thaliana ?
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O 83.5% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana ?
-
 ?
3.1.3.2 polyphosphate + H2O 72% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 additional information two proteins of 52000 Da and 63000 Da are detected by SDS-PAGE Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5
-
-
 Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.2 Arabidopsis thaliana isoelectric focusing, pH-gradient: pH 3.5-10
-
 5.9