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Literature summary extracted from

  • Cummins, I.; Burnet, M.; Edwards, R.
    Biochemical characterization of esterases active in hydrolysing xenobiotics in wheat and competing weeds (2001), Physiol. Plant., 113, 477-485.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 4.2
-
 4-nitrophenyl acetate pH 7.2, 37°C Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.2 45000
-
 gel filtration Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Alopecurus myosuroides
-
 2 populations, one of which, Rothamsted, is suceptible to herbicides, while the other, Peldon, shows cross-resistance to multiple classes of herbicides
-
3.1.1.2 Avena fatua
-
-
-
3.1.1.2 Triticum aestivum
-
 cv. Hunter
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2 7300fold Triticum aestivum
3.1.1.2 partially Avena fatua
3.1.1.2 partially Alopecurus myosuroides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.2 shoot
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.2 0.00014
-
 partially purified enzyme, substrate diclofop methyl Avena fatua
3.1.1.2 0.00024
-
 partially purified enzyme, substrate binapacryl Triticum aestivum
3.1.1.2 0.00031
-
 partially purified enzyme, substrate binapacryl, population Rothamsted Alopecurus myosuroides
3.1.1.2 0.00043
-
 partially purified enzyme, substrate binapacryl, population Peldon Alopecurus myosuroides
3.1.1.2 0.00049
-
 partially purified enzyme, substrate binapacryl Avena fatua
3.1.1.2 0.00066
-
 partially purified enzyme, substrate diclofop methyl Alopecurus myosuroides
3.1.1.2 0.0048
-
 partially purified enzyme, substrate bromoxynil octanoate, population Peldon Alopecurus myosuroides
3.1.1.2 0.0056
-
 partially purified enzyme, substrate bromoxynil octanoate Triticum aestivum
3.1.1.2 0.006
-
 purified enzyme, substrate binapacryl Triticum aestivum
3.1.1.2 0.0061
-
 partially purified enzyme, substrate bromoxynil octanoate, population Rothamsted Alopecurus myosuroides
3.1.1.2 0.007
-
 partially purified enzyme, substrate bromoxynil octanoate Avena fatua
3.1.1.2 0.03
-
 partially purified enzyme, substrate 4-nitrophenyl acetate Avena fatua
3.1.1.2 0.04
-
 partially purified enzyme, substrate 4-nitrophenyl acetate Alopecurus myosuroides
3.1.1.2 0.08
-
 partially purified enzyme, substrate 4-nitrophenyl acetate Triticum aestivum
3.1.1.2 0.19
-
 purified enzyme, substrate bromoxynil octanoate Triticum aestivum
3.1.1.2 4.18
-
 purified enzyme, substrate 4-nitrophenyl butyrate Triticum aestivum
3.1.1.2 77.9
-
 purified enzyme, substrate 4-nitrophenyl acetate Triticum aestivum
3.1.1.2 159.2
-
 purified enzyme, substrate beta-naphthyl acetate Triticum aestivum
3.1.1.2 170.7
-
 purified enzyme, substrate alpha-naphthyl acetate Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 4-nitrophenyl acetate + H2O
-
 Triticum aestivum 4-nitrophenol + acetate
-
 ?
3.1.1.2 4-nitrophenyl acetate + H2O
-
 Avena fatua 4-nitrophenol + acetate
-
 ?
3.1.1.2 4-nitrophenyl acetate + H2O
-
 Alopecurus myosuroides 4-nitrophenol + acetate
-
 ?
3.1.1.2 alpha-naphthyl acetate + H2O
-
 Triticum aestivum alpha-naphthol + acetate
-
 ?
3.1.1.2 alpha-naphthyl acetate + H2O
-
 Avena fatua alpha-naphthol + acetate
-
 ?
3.1.1.2 alpha-naphthyl acetate + H2O
-
 Alopecurus myosuroides alpha-naphthol + acetate
-
 ?
3.1.1.2 binapacryl + H2O
-
 Triticum aestivum ?
-
 ?
3.1.1.2 binapacryl + H2O
-
 Avena fatua ?
-
 ?
3.1.1.2 binapacryl + H2O
-
 Alopecurus myosuroides ?
-
 ?
3.1.1.2 bromoxynil octanoate + H2O
-
 Triticum aestivum ?
-
 ?
3.1.1.2 bromoxynil octanoate + H2O
-
 Avena fatua ?
-
 ?
3.1.1.2 bromoxynil octanoate + H2O
-
 Alopecurus myosuroides ?
-
 ?
3.1.1.2 diclofop methyl + H2O
-
 Triticum aestivum ?
-
 ?
3.1.1.2 diclofop methyl + H2O
-
 Avena fatua ?
-
 ?
3.1.1.2 diclofop methyl + H2O
-
 Alopecurus myosuroides ?
-
 ?
3.1.1.2 additional information no activity with 4-nitrophenyl caprylate and diclofop methyl Triticum aestivum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.2 monomer 1 * 45000, SDS-PAGE Triticum aestivum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.2 37
-
 assay at Triticum aestivum
3.1.1.2 37
-
 assay at Avena fatua
3.1.1.2 37
-
 assay at Alopecurus myosuroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.2 7.2
-
 assay at Triticum aestivum
3.1.1.2 7.2
-
 assay at Avena fatua
3.1.1.2 7.2
-
 assay at Alopecurus myosuroides

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.2 Triticum aestivum isoelectric focusing 4.7 4.6