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Literature summary extracted from

  • Chinthapalli, B.; Raghavan, C.; Blasing, O.; Westhoff, P.; Raghavendra, A.S.
    Phosphoenolpyruvate carboxylase purified from leaves of C3, C4, and C3-C4 intermediate species of Alternanthera: Properties at limiting and saturating bicarbonate (2000), Photosynthetica, 38, 415-419.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.49 D-Glucose-6-phosphate
-
 Alternanthera sessilis
4.1.1.49 D-Glucose-6-phosphate
-
 Alternanthera pungens
4.1.1.49 D-Glucose-6-phosphate
-
 Alternanthera ficoides
4.1.1.49 D-Glucose-6-phosphate
-
 Alternanthera ficoidea

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.49 malate
-
 Alternanthera ficoidea
4.1.1.49 malate
-
 Alternanthera ficoides
4.1.1.49 malate
-
 Alternanthera pungens
4.1.1.49 malate
-
 Alternanthera sessilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.49 0.05
-
 phosphoenolpyruvate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera sessilis
4.1.1.49 0.07
-
 phosphoenolpyruvate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera sessilis
4.1.1.49 0.11
-
 phosphoenolpyruvate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera ficoides
4.1.1.49 0.15
-
 phosphoenolpyruvate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera ficoidea
4.1.1.49 0.2
-
 phosphoenolpyruvate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera ficoides
4.1.1.49 0.28
-
 phosphoenolpyruvate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera pungens
4.1.1.49 0.29
-
 phosphoenolpyruvate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera ficoidea
4.1.1.49 0.62
-
 phosphoenolpyruvate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera pungens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.49 Mg2+ 0.05 mM bicarbonate, Km 0.20 mM, 10 mM bicarbonate Km 0.04 mM Alternanthera sessilis
4.1.1.49 Mg2+ 0.05 mM bicarbonate, Km 0.44 mM, 10 mM bicarbonate Km 0.14 mM Alternanthera ficoides
4.1.1.49 Mg2+ 0.05 mM bicarbonate, Km 0.50 mM, 10 mM bicarbonate Km 0.15 mM Alternanthera ficoidea
4.1.1.49 Mg2+ 0.05 mM bicarbonate, Km 1.20 mM, 10 mM bicarbonate Km 0.42 mM Alternanthera pungens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.49 ATP + oxaloacetate Alternanthera sessilis
-
 ADP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 ATP + oxaloacetate Alternanthera pungens
-
 ADP + phosphoenolpyruvate + CO2
-
 r
4.1.1.49 ATP + oxaloacetate Alternanthera ficoides
-
 ADP + phosphoenolpyruvate + CO2
-
 r
4.1.1.49 ATP + oxaloacetate Alternanthera ficoidea
-
 ADP + phosphoenolpyruvate + CO2
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.49 Alternanthera ficoidea
-
-
-
4.1.1.49 Alternanthera ficoides
-
-
-
4.1.1.49 Alternanthera pungens
-
-
-
4.1.1.49 Alternanthera sessilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.49
-
 Alternanthera sessilis
4.1.1.49
-
 Alternanthera pungens
4.1.1.49
-
 Alternanthera ficoides
4.1.1.49
-
 Alternanthera ficoidea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.49 40.5
-
-
 Alternanthera pungens

Storage Stability

EC Number Storage Stability Organism
4.1.1.49 -196°C, stored in 50% glycerol in liquid nitrogen, stable for more than 3 months Alternanthera pungens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.49 ATP + oxaloacetate
-
 Alternanthera sessilis ADP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.49 ATP + oxaloacetate
-
 Alternanthera pungens ADP + phosphoenolpyruvate + CO2
-
 r
4.1.1.49 ATP + oxaloacetate
-
 Alternanthera ficoides ADP + phosphoenolpyruvate + CO2
-
 r
4.1.1.49 ATP + oxaloacetate
-
 Alternanthera ficoidea ADP + phosphoenolpyruvate + CO2
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.1.49 PEPC
-
 Alternanthera sessilis
4.1.1.49 PEPC
-
 Alternanthera pungens
4.1.1.49 PEPC
-
 Alternanthera ficoides
4.1.1.49 PEPC
-
 Alternanthera ficoidea
4.1.1.49 phosphoenolpyruvate carboxykinase
-
 Alternanthera sessilis
4.1.1.49 phosphoenolpyruvate carboxykinase
-
 Alternanthera pungens
4.1.1.49 phosphoenolpyruvate carboxykinase
-
 Alternanthera ficoides
4.1.1.49 phosphoenolpyruvate carboxykinase
-
 Alternanthera ficoidea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.49 0.004
-
 malate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera sessilis
4.1.1.49 0.013
-
 malate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera ficoides
4.1.1.49 0.013
-
 malate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera sessilis
4.1.1.49 0.014
-
 malate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera ficoidea
4.1.1.49 0.015
-
 malate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera pungens
4.1.1.49 0.046
-
 malate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera ficoides
4.1.1.49 0.052
-
 malate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera ficoidea
4.1.1.49 0.07
-
 malate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera pungens