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Literature summary extracted from

  • Usui, S.; Kubota, M.; Iguchi, K.; Kiho, T.; Sugiyama, T.; Katagiri, Y.; Hirano, K.
    Sialic acid 9-O-acetylesterase catalyzes the hydrolyzing reaction from alacepril to deacetylalacepril (2003), Pharm. Res., 20, 1309-1316.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.53 Alpha-naphthyl acetate competitive inhibitor Rattus norvegicus
3.1.1.53 Cu2+ complete inhibition at 1 mM Rattus norvegicus
3.1.1.53 diisopropyl fluorophosphates complete inhibition at 1 mM Rattus norvegicus
3.1.1.53 Hg2+ complete inhibition at 1 mM Rattus norvegicus
3.1.1.53 phenylmethylsulfonyl fluoride complete inhibition at 10 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.53 0.652
-
 spironolactone pH 7.5, 37°C Rattus norvegicus
3.1.1.53 1.28
-
 acetyl-CoA pH 7.5, 37°C Rattus norvegicus
3.1.1.53 1.71
-
 Alpha-naphthyl acetate pH 7.5, 37°C Rattus norvegicus
3.1.1.53 22
-
 alacepril pH 7.5, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.53 cytosol
-
 Rattus norvegicus 5829
-
3.1.1.53 microsome only little activity Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.53 29000
-
 1 * 29000 + 1 * 36000, SDS-PAGE Rattus norvegicus
3.1.1.53 36000
-
 1 * 29000 + 1 * 36000, SDS-PAGE Rattus norvegicus
3.1.1.53 60000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.53 Rattus norvegicus
-
 male Sprague-Dawley
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.53 0.695
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.53 acetyl-CoA + H2O
-
 Rattus norvegicus CoA + acetate
-
 ?
3.1.1.53 alacepril + H2O
-
 Rattus norvegicus deacetylalacepril + acetate
-
 ?
3.1.1.53 alpha-naphthyl acetate + H2O
-
 Rattus norvegicus alpha-naphthol + acetate
-
 ?
3.1.1.53 spironolactone + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.53 dimer 1 * 29000 + 1 * 36000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.53 25
-
 room temperature, complete inactivation in the presence of 10 mM 2-mercaptoethanol after 8 h Rattus norvegicus