BRENDA - Enzyme Database

An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli

Santoro, S.W.; Anderson, J.C.; Lakshman, V.; Schultz, P.G.; Nucleic Acids Res. 31, 6700-6709 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Aeropyrum pernix
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Archaeoglobus fulgidus
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Methanocaldococcus jannaschii
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Methanococcus thermoautotrophicum
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Methanosarcina mazei
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Pyrococcus horikoshii
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Saccharolobus solfataricus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Aeropyrum pernix
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Archaeoglobus fulgidus
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Methanocaldococcus jannaschii
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Methanococcus thermoautotrophicum
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Methanosarcina mazei
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Pyrococcus horikoshii
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Saccharolobus solfataricus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
Saccharolobus solfataricus
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Saccharolobus solfataricus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Methanocaldococcus jannaschii
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Methanocaldococcus jannaschii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Archaeoglobus fulgidus
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Archaeoglobus fulgidus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Pyrococcus horikoshii
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Pyrococcus horikoshii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Methanosarcina mazei
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Methanosarcina mazei
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Aeropyrum pernix
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Aeropyrum pernix
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Methanococcus thermoautotrophicum
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Methanococcus thermoautotrophicum
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Aeropyrum pernix
-
-
-
6.1.1.17
Archaeoglobus fulgidus
-
-
-
6.1.1.17
Methanocaldococcus jannaschii
-
-
-
6.1.1.17
Methanococcus thermoautotrophicum
-
-
-
6.1.1.17
Methanosarcina mazei
-
-
-
6.1.1.17
Pyrococcus horikoshii
-
-
-
6.1.1.17
Saccharolobus solfataricus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Saccharolobus solfataricus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Saccharolobus solfataricus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Methanocaldococcus jannaschii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanocaldococcus jannaschii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Archaeoglobus fulgidus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Archaeoglobus fulgidus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Pyrococcus horikoshii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Pyrococcus horikoshii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Methanosarcina mazei
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanosarcina mazei
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Aeropyrum pernix
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Aeropyrum pernix
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Methanococcus thermoautotrophicum
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanococcus thermoautotrophicum
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Saccharolobus solfataricus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Saccharolobus solfataricus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Methanocaldococcus jannaschii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanocaldococcus jannaschii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Archaeoglobus fulgidus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Archaeoglobus fulgidus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Pyrococcus horikoshii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Pyrococcus horikoshii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Methanosarcina mazei
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanosarcina mazei
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Aeropyrum pernix
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Aeropyrum pernix
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Methanococcus thermoautotrophicum
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanococcus thermoautotrophicum
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Saccharolobus solfataricus
?
-
653391
Saccharolobus solfataricus
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Methanocaldococcus jannaschii
?
-
653391
Methanocaldococcus jannaschii
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Archaeoglobus fulgidus
?
-
653391
Archaeoglobus fulgidus
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Pyrococcus horikoshii
?
-
653391
Pyrococcus horikoshii
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Methanosarcina mazei
?
-
653391
Methanosarcina mazei
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Aeropyrum pernix
?
-
653391
Aeropyrum pernix
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Methanococcus thermoautotrophicum
?
-
653391
Methanococcus thermoautotrophicum
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.17
ATP
-
Aeropyrum pernix
6.1.1.17
ATP
-
Archaeoglobus fulgidus
6.1.1.17
ATP
-
Methanocaldococcus jannaschii
6.1.1.17
ATP
-
Methanococcus thermoautotrophicum
6.1.1.17
ATP
-
Methanosarcina mazei
6.1.1.17
ATP
-
Pyrococcus horikoshii
6.1.1.17
ATP
-
Saccharolobus solfataricus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Aeropyrum pernix
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Archaeoglobus fulgidus
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Methanocaldococcus jannaschii
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Methanococcus thermoautotrophicum
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Methanosarcina mazei
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Pyrococcus horikoshii
6.1.1.17
cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants
Saccharolobus solfataricus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.17
ATP
-
Aeropyrum pernix
6.1.1.17
ATP
-
Archaeoglobus fulgidus
6.1.1.17
ATP
-
Methanocaldococcus jannaschii
6.1.1.17
ATP
-
Methanococcus thermoautotrophicum
6.1.1.17
ATP
-
Methanosarcina mazei
6.1.1.17
ATP
-
Pyrococcus horikoshii
6.1.1.17
ATP
-
Saccharolobus solfataricus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Aeropyrum pernix
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Archaeoglobus fulgidus
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Methanocaldococcus jannaschii
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Methanococcus thermoautotrophicum
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Methanosarcina mazei
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Pyrococcus horikoshii
6.1.1.17
additional information
design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28
Saccharolobus solfataricus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
Saccharolobus solfataricus
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Saccharolobus solfataricus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Methanocaldococcus jannaschii
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Methanocaldococcus jannaschii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Archaeoglobus fulgidus
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Archaeoglobus fulgidus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Pyrococcus horikoshii
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Pyrococcus horikoshii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Methanosarcina mazei
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Methanosarcina mazei
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Aeropyrum pernix
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Aeropyrum pernix
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Methanococcus thermoautotrophicum
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Methanococcus thermoautotrophicum
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Saccharolobus solfataricus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Saccharolobus solfataricus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Methanocaldococcus jannaschii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanocaldococcus jannaschii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Archaeoglobus fulgidus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Archaeoglobus fulgidus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Pyrococcus horikoshii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Pyrococcus horikoshii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Methanosarcina mazei
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanosarcina mazei
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Aeropyrum pernix
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Aeropyrum pernix
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653391
Methanococcus thermoautotrophicum
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanococcus thermoautotrophicum
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Saccharolobus solfataricus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Saccharolobus solfataricus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Methanocaldococcus jannaschii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanocaldococcus jannaschii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Archaeoglobus fulgidus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Archaeoglobus fulgidus
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Pyrococcus horikoshii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Pyrococcus horikoshii
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Methanosarcina mazei
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanosarcina mazei
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Aeropyrum pernix
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Aeropyrum pernix
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
wild-type tRNAGlu and tRNA AE(GU)
653391
Methanococcus thermoautotrophicum
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653391
Methanococcus thermoautotrophicum
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Saccharolobus solfataricus
?
-
653391
Saccharolobus solfataricus
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Methanocaldococcus jannaschii
?
-
653391
Methanocaldococcus jannaschii
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Archaeoglobus fulgidus
?
-
653391
Archaeoglobus fulgidus
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Pyrococcus horikoshii
?
-
653391
Pyrococcus horikoshii
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Methanosarcina mazei
?
-
653391
Methanosarcina mazei
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Aeropyrum pernix
?
-
653391
Aeropyrum pernix
?
6.1.1.17
additional information
no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC)
653391
Methanococcus thermoautotrophicum
?
-
653391
Methanococcus thermoautotrophicum
?