Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Santoro, S.W.; Anderson, J.C.; Lakshman, V.; Schultz, P.G.
    An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli (2003), Nucleic Acids Res., 31, 6700-6709.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.17 cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants Saccharolobus solfataricus
6.1.1.17 cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants Methanocaldococcus jannaschii
6.1.1.17 cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants Archaeoglobus fulgidus
6.1.1.17 cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants Pyrococcus horikoshii
6.1.1.17 cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants Methanosarcina mazei
6.1.1.17 cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants Aeropyrum pernix
6.1.1.17 cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants Methanococcus thermoautotrophicum

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.17 additional information design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 Saccharolobus solfataricus
6.1.1.17 additional information design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 Methanocaldococcus jannaschii
6.1.1.17 additional information design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 Archaeoglobus fulgidus
6.1.1.17 additional information design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 Pyrococcus horikoshii
6.1.1.17 additional information design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 Methanosarcina mazei
6.1.1.17 additional information design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 Aeropyrum pernix
6.1.1.17 additional information design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 Methanococcus thermoautotrophicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGlu Saccharolobus solfataricus
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu Methanocaldococcus jannaschii
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu Archaeoglobus fulgidus
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu Pyrococcus horikoshii
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu Methanosarcina mazei
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu Aeropyrum pernix
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu Methanococcus thermoautotrophicum
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Aeropyrum pernix
-
-
-
6.1.1.17 Archaeoglobus fulgidus
-
-
-
6.1.1.17 Methanocaldococcus jannaschii
-
-
-
6.1.1.17 Methanococcus thermoautotrophicum
-
-
-
6.1.1.17 Methanosarcina mazei
-
-
-
6.1.1.17 Pyrococcus horikoshii
-
-
-
6.1.1.17 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Saccharolobus solfataricus AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Methanocaldococcus jannaschii AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Archaeoglobus fulgidus AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Pyrococcus horikoshii AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Methanosarcina mazei AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Aeropyrum pernix AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Methanococcus thermoautotrophicum AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu wild-type tRNAGlu and tRNA AE(GU) Saccharolobus solfataricus AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu wild-type tRNAGlu and tRNA AE(GU) Methanocaldococcus jannaschii AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu wild-type tRNAGlu and tRNA AE(GU) Archaeoglobus fulgidus AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu wild-type tRNAGlu and tRNA AE(GU) Pyrococcus horikoshii AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu wild-type tRNAGlu and tRNA AE(GU) Methanosarcina mazei AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu wild-type tRNAGlu and tRNA AE(GU) Aeropyrum pernix AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu wild-type tRNAGlu and tRNA AE(GU) Methanococcus thermoautotrophicum AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 additional information no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) Saccharolobus solfataricus ?
-
 ?
6.1.1.17 additional information no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) Methanocaldococcus jannaschii ?
-
 ?
6.1.1.17 additional information no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) Archaeoglobus fulgidus ?
-
 ?
6.1.1.17 additional information no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) Pyrococcus horikoshii ?
-
 ?
6.1.1.17 additional information no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) Methanosarcina mazei ?
-
 ?
6.1.1.17 additional information no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) Aeropyrum pernix ?
-
 ?
6.1.1.17 additional information no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) Methanococcus thermoautotrophicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.17 GluRS
-
 Methanocaldococcus jannaschii
6.1.1.17 Glutamate--tRNA ligase
-
 Methanocaldococcus jannaschii
6.1.1.17 Glutamate-tRNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.17 Glutamic acid translase
-
 Methanocaldococcus jannaschii
6.1.1.17 Glutamic acid tRNA ligase
-
 Methanocaldococcus jannaschii
6.1.1.17 Glutamyl tRNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.17 Glutamyl-transfer ribonucleate synthetase
-
 Methanocaldococcus jannaschii
6.1.1.17 Glutamyl-transfer ribonucleic acid synthetase
-
 Methanocaldococcus jannaschii
6.1.1.17 Glutamyl-transfer RNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.17 Glutamyl-tRNA synthetase
-
 Saccharolobus solfataricus
6.1.1.17 Glutamyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.17 Glutamyl-tRNA synthetase
-
 Archaeoglobus fulgidus
6.1.1.17 Glutamyl-tRNA synthetase
-
 Pyrococcus horikoshii
6.1.1.17 Glutamyl-tRNA synthetase
-
 Methanosarcina mazei
6.1.1.17 Glutamyl-tRNA synthetase
-
 Aeropyrum pernix
6.1.1.17 Glutamyl-tRNA synthetase
-
 Methanococcus thermoautotrophicum
6.1.1.17 P85
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.17 ATP
-
 Saccharolobus solfataricus
6.1.1.17 ATP
-
 Methanocaldococcus jannaschii
6.1.1.17 ATP
-
 Archaeoglobus fulgidus
6.1.1.17 ATP
-
 Pyrococcus horikoshii
6.1.1.17 ATP
-
 Methanosarcina mazei
6.1.1.17 ATP
-
 Aeropyrum pernix
6.1.1.17 ATP
-
 Methanococcus thermoautotrophicum