Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tardif, K.D.; Horowitz, J.
    Transfer RNA determinants for translational editing by Escherichia coli valyl-tRNA synthetase (2002), Nucleic Acids Res., 30, 2538-2545.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.9 additional information stimulatory effects of diverse tRNA variants on the editing activity, overview Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.9 additional information substitution of base 75 of C by U or A leads to effective stimulation of the editing activity at lower rates, substitution with G leads to reduction of the editing activity by 95%, mutational exchange of the discriminator base A73 to U73 or C73 does not alter enzyme activity but exchange to G73 stimulates editing activity of the catalytically inactive mutant Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.9 ATP + L-valine + tRNAVal Escherichia coli
-
 AMP + diphosphate + L-valyl-tRNAVal
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.9 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.9 ATP + L-valine + tRNAVal = AMP + diphosphate + L-valyl-tRNAVal substrate binding mechanism, bases A73 and C75 are involved in the tRNA substrate recognition and editing reaction Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.9 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.9 ATP + L-threonine + tRNAVal 1 step performance of an originally two-step reaction, the enzyme can hardly differentiate between the cognate amino acid valine and others, especially threonine, to minimize misaminoacetylation the enzyme performs a proofreading, socalled editing reaction at a second active site, which is dependent on the presence of cognate tRNAVal whose 3'-end is involved in the editing reaction, a majority of editing by the enzyme entails prior charging of the tRNA, misacylated tRNA is a transient intermediate in the editing reaction Escherichia coli ?
-
 ?
6.1.1.9 ATP + L-valine + tRNAVal
-
 Escherichia coli AMP + diphosphate + L-valyl-tRNAVal
-
 ?
6.1.1.9 ATP + L-valine + tRNAVal two-step reaction, the enzyme can hardly differentiate between the cognate amino acid valine and others, especially threonine, to minimize misaminoacetylation the enzyme performs a proofreading, socalled editing reaction at a second active site, which is dependent on the presence of cognate tRNAVal whose 3'-end is involved in the editing reaction, a majority of editing by the enzyme entails prior charging of the tRNA, misacylated tRNA is a transient intermediate in the editing reaction Escherichia coli AMP + diphosphate + L-valyl-tRNAVal
-
 ?
6.1.1.9 L-threonyl-tRNAVal substrate bound to the enzyme, intermediate in the posttransfer editing reaction Escherichia coli L-threonine + tRNAVal
-
 ir
6.1.1.9 L-threonyl-tRNAVal-AMP substrate bound to the enzyme, intermediate in the pretransfer editing reaction Escherichia coli L-threonine + tRNAVal + AMP
-
 ir
6.1.1.9 additional information substrate specificity with diverse Escherichia coli tRNAs and tRNA mutants in editing and aminoacylation reaction, overview Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.9 G7a
-
 Escherichia coli
6.1.1.9 Synthetase, valyl-transfer ribonucleate
-
 Escherichia coli
6.1.1.9 Valine transfer ribonucleate ligase
-
 Escherichia coli
6.1.1.9 Valine translase
-
 Escherichia coli
6.1.1.9 Valine--tRNA ligase
-
 Escherichia coli
6.1.1.9 ValRS
-
 Escherichia coli
6.1.1.9 Valyl transfer ribonucleic acid synthetase
-
 Escherichia coli
6.1.1.9 Valyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.9 Valyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.9 Valyl-tRNA ligase
-
 Escherichia coli
6.1.1.9 Valyl-tRNA synthetase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.9 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.9 7.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.9 ATP
-
 Escherichia coli