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Literature summary extracted from

  • Sriskanda, V.; Shuman, S.
    Mutational analysis of Chlorella virus DNA ligase: catalytic roles of domain I and motif VI (1998), Nucleic Acids Res., 26, 4618-4625.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expression in Escherichia coli Chlorella virus

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.1 Cdel5 mutant lacking the C-terminal pentapeptide HEEDR of motif VI, 1% of wild-type DNA ligase activity Chlorella virus
6.5.1.1 D297A 14% of wild-type DNA ligase activity Chlorella virus
6.5.1.1 E295A 90% of wild-type DNA ligase activity Chlorella virus
6.5.1.1 E296A 52% of wild-type DNA ligase activity Chlorella virus
6.5.1.1 F286A 13% of wild-type DNA ligase activity Chlorella virus
6.5.1.1 F289A 42% of wild-type DNA ligase activity Chlorella virus
6.5.1.1 H294A 92% of wild-type DNA ligase activity Chlorella virus
6.5.1.1 P284A 53% of wild-type DNA ligase activity Chlorella virus
6.5.1.1 P287A 59% of wild-type DNA ligase activity Chlorella virus
6.5.1.1 R285A 26% of wild-type DNA ligase activity Chlorella virus
6.5.1.1 R293A 30% of wild-type DNA ligase activity Chlorella virus
6.5.1.1 R298A 85% of wild-type DNA ligase activity Chlorella virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Chlorella virus
-
 AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Chlorella virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 recombinant DNA ligase Chlorella virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Chlorella virus AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.1 More 298 amino acid Chlorella virus PBCV-1 ligase is the smallest eukaryotic DNA ligase known Chlorella virus

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 PBCV-1 DNA ligase
-
 Chlorella virus