BRENDA - Enzyme Database

Regulation of phospholipase C activity by calcium ions and guanine nucleotide in the normoxic cat carotid body

Strosznajder, R.P.; Pokorski, M.; Neurochem. Res. 25, 739-743 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
Ca2+
required, isoform acting on inositol-phosphatidylinositol
Felis catus
3.1.4.3
GTPgammaS
isoform acting on inositol-phosphatidylinositol-4,5-bisphosphate
Felis catus
3.1.4.11
Ca2+
the enzyme acting on PtdIns is Ca2+-dependent, in contrary to that acting on PtdIns(4,5)P2 which remains active in the presence of 10 mM EGTA
Felis catus
3.1.4.11
Carbachol
small stimulatory effect of about 30% on the enzyme acting on PtdIns(4,5)P2. GTPgammaS enhances this effect
Felis catus
3.1.4.11
dopamine
small stimulatory effect of about 30% on the enzyme acting on PtdIns(4,5)P2. GTPgammaS enhances this effect
Felis catus
3.1.4.11
GTPgammaS
PtdIns(4,5)P2-PLC is stimulated by GTPgammaS. Enzyme acting on PtdIns is not activated by GTPgS
Felis catus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
EDTA
isoform acting on inositol-phosphatidylinositol
Felis catus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.11
additional information
Felis catus
the enzymes acting against [3H] inositol-phosphatidylinositol (PtdIns) and [3H] inositolphosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] in the cat carotid bodies (CB) are investigated using labelled phospholipids as a source of the substrate. Analysis of water soluble inositol metabolites indicates that PtdIns is degraded exclusively by the enzyme since the inositolmonophosphate (IP1) is detected as a single product. In case of PtdIns(4,5)P2 hydrolysis by carotid bodies (CB) enzymes there are all 3 inositol phosphates: IP3, IP2, IP1
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Felis catus
-
two isozymes
-
3.1.4.11
Felis catus
-
adult
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.3
carotid body
-
Felis catus
-
3.1.4.11
carotid body
the carotid bodies (CB) are a paired chemoreceptor organ located at the bifurcation of the common carotid arteries
Felis catus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
phosphatidylinositol + H2O
Ca2+-dependent activity
653351
Felis catus
inositol + phosphatidate
-
653351
Felis catus
?
3.1.4.3
phosphatidylinositol 4,5-bisphosphate + H2O
Ca2+-independent activity
653351
Felis catus
inositol 4,5-bisphosphate + phosphatidate
-
653351
Felis catus
?
3.1.4.11
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
653351
Felis catus
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
-
-
?
3.1.4.11
additional information
the enzymes acting against [3H] inositol-phosphatidylinositol (PtdIns) and [3H] inositolphosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] in the cat carotid bodies (CB) are investigated using labelled phospholipids as a source of the substrate. Analysis of water soluble inositol metabolites indicates that PtdIns is degraded exclusively by the enzyme since the inositolmonophosphate (IP1) is detected as a single product. In case of PtdIns(4,5)P2 hydrolysis by carotid bodies (CB) enzymes there are all 3 inositol phosphates: IP3, IP2, IP1
653351
Felis catus
?
-
-
-
-
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
Ca2+
required, isoform acting on inositol-phosphatidylinositol
Felis catus
3.1.4.3
GTPgammaS
isoform acting on inositol-phosphatidylinositol-4,5-bisphosphate
Felis catus
3.1.4.11
Ca2+
the enzyme acting on PtdIns is Ca2+-dependent, in contrary to that acting on PtdIns(4,5)P2 which remains active in the presence of 10 mM EGTA
Felis catus
3.1.4.11
Carbachol
small stimulatory effect of about 30% on the enzyme acting on PtdIns(4,5)P2. GTPgammaS enhances this effect
Felis catus
3.1.4.11
dopamine
small stimulatory effect of about 30% on the enzyme acting on PtdIns(4,5)P2. GTPgammaS enhances this effect
Felis catus
3.1.4.11
GTPgammaS
PtdIns(4,5)P2-PLC is stimulated by GTPgammaS. Enzyme acting on PtdIns is not activated by GTPgS
Felis catus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
EDTA
isoform acting on inositol-phosphatidylinositol
Felis catus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.11
additional information
Felis catus
the enzymes acting against [3H] inositol-phosphatidylinositol (PtdIns) and [3H] inositolphosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] in the cat carotid bodies (CB) are investigated using labelled phospholipids as a source of the substrate. Analysis of water soluble inositol metabolites indicates that PtdIns is degraded exclusively by the enzyme since the inositolmonophosphate (IP1) is detected as a single product. In case of PtdIns(4,5)P2 hydrolysis by carotid bodies (CB) enzymes there are all 3 inositol phosphates: IP3, IP2, IP1
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.3
carotid body
-
Felis catus
-
3.1.4.11
carotid body
the carotid bodies (CB) are a paired chemoreceptor organ located at the bifurcation of the common carotid arteries
Felis catus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
phosphatidylinositol + H2O
Ca2+-dependent activity
653351
Felis catus
inositol + phosphatidate
-
653351
Felis catus
?
3.1.4.3
phosphatidylinositol 4,5-bisphosphate + H2O
Ca2+-independent activity
653351
Felis catus
inositol 4,5-bisphosphate + phosphatidate
-
653351
Felis catus
?
3.1.4.11
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
653351
Felis catus
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
-
-
?
3.1.4.11
additional information
the enzymes acting against [3H] inositol-phosphatidylinositol (PtdIns) and [3H] inositolphosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] in the cat carotid bodies (CB) are investigated using labelled phospholipids as a source of the substrate. Analysis of water soluble inositol metabolites indicates that PtdIns is degraded exclusively by the enzyme since the inositolmonophosphate (IP1) is detected as a single product. In case of PtdIns(4,5)P2 hydrolysis by carotid bodies (CB) enzymes there are all 3 inositol phosphates: IP3, IP2, IP1
653351
Felis catus
?
-
-
-
-