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Literature summary extracted from

  • Blanco, P.R.; Brewer, L.R.; Corzett, M.; Balhorn, R.; Yeh, Y.; Kowalczykowski, S.C.; Baskin, R.J.
    Processive translocation and DNA unwinding by individual RecBCD enzyme molecules (2001), Nature, 409, 374-378.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.5 double-stranded DNA Escherichia coli ATP-dependent, unwinding of DNA molecule single-stranded DNA
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Organism

EC Number Organism UniProt Comment Textmining
3.1.11.5 Escherichia coli
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.5 double-stranded DNA ATP-dependent, unwinding of DNA molecule Escherichia coli single-stranded DNA
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Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.11.5 23 37 The rate of unwinding in 1 mM ATP increases twofold when the temperature is raised from 23°C to 37°C. Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.11.5 additional information
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 additional information The corrected unwinding rate is 443 base pairs 1/sec, which is due to a single molecule of enzyme that bound to the free double-stranded DNA end opposite the bead, and both translocates and unwinds the DNA in an ATP-dependent manner once the DNA entered the ATP channel. The rate of unwinding increases with increasing ATP concentration and increasing temperature Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
3.1.11.5 ATP the unwinding of Escherichia coli