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Literature summary extracted from

  • Rasmussen, H.B.; Branner, S.; Wiberg, F.C.; Wagtmann, N.
    Crystal structure of human dipeptidyl peptidase IV/CD26 in complex with a substrate analog (2003), Nat. Struct. Biol., 10, 19-25.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.5 expression of a soluble enzyme form, which comprises amino acid residues 39-766 and is secreted to the medium, in Hi5 insect cells via baculovirus infection Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.14.5 40 mg/ml extracellular enzyme region complexed with inhibitor valine-pyrrolidine in 20 mM Tris, pH 8.0, ans 25 mM NaCl, sitting drop vapour diffusion method, mixed with equal volume of reservoir solution containing 0.3 M sodium acetate, 17-18% w/v PEG 4000, 0.1 M Tris, pH 8.0, 2-3 days, X-ray diffraction structure determination and analysis at 2.5 A resolution Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 additional information alpha/beta-hydrolase domain and beta-propeller domain both participate in inhibitor binding Homo sapiens
3.4.14.5 valine-pyrrolidine
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.5 membrane type II transmembrane enzyme Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.5 84400
-
 non-glycosylated enzyme, calculated from amino acid sequence Homo sapiens
3.4.14.5 93500
-
 recombinant soluble enzyme, MALDI mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.14.5 additional information Homo sapiens contributes to the regulation of various physiological processes, including blood sugar homeostasis, by cleaving peptide hormones, chemokines, and neuropeptides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Homo sapiens P27487 multifunctional enzyme
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.14.5 glycoprotein 11% glycosylation Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5 recombinant soluble enzyme from insect cell culture supernatant to homogeneity Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.14.5 release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline enzyme is a serine protease, catalytic and substrate binding mechanism, active site structure, catalytic triad Ser630, Asp708, His740 Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Homo sapiens Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.5 additional information contributes to the regulation of various physiological processes, including blood sugar homeostasis, by cleaving peptide hormones, chemokines, and neuropeptides Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.5 dimer homodimer, crystal structure Homo sapiens
3.4.14.5 More the catalytic site is located in a large cavity formed between the alpha/beta-hydrolase domain and an eight-bladed beta-propeller domain Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.14.5 CD26
-
 Homo sapiens
3.4.14.5 dipeptidyl peptidase IV
-
 Homo sapiens
3.4.14.5 DPP-IV
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 7.4
-
 assay at Homo sapiens