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Literature summary extracted from

  • Jacobs, T.; Bruchhaus, I.; Dankebar, T.; Tannich, E.; Leippe, M.
    Isolation and molecular characterization of a surface-bound proteinase of Entamoeba histolytica (1998), Mol. Microbiol., 27, 169-276.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.35 Liposomes association increases activity about 2fold Entamoeba histolytica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.35 DNA and amino acid sequence determination Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.35 iodoacetamide
-
 Entamoeba histolytica
3.4.22.35 p-hydroxymercuribenzoate
-
 Entamoeba histolytica
3.4.22.35 trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane i.e. E64 Entamoeba histolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.35 extracellular bound to the host cell surface Entamoeba histolytica
-
-
3.4.22.35 membrane associated by hydrophobic interaction Entamoeba histolytica 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.35 protein + H2O Entamoeba histolytica enzyme is involved in pathogenic destruction of host tissue by degradation of extracellular matrix proteins peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.35 Entamoeba histolytica Q95030 enzyme is termed CP5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.35 to homogeneity Entamoeba histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.35 trophozoite
-
 Entamoeba histolytica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.35 470
-
 purified enzyme Entamoeba histolytica
3.4.22.35 890
-
 purified enzyme associated to liposomes Entamoeba histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.35 benzyloxycarbonyl-L-arginyl-L-arginine 4-nitroanilide + H2O
-
 Entamoeba histolytica benzyloxycarbonyl-L-arginyl-L-arginine + 4-nitroaniline
-
 ?
3.4.22.35 complement factor C3 + H2O
-
 Entamoeba histolytica peptides product determination ?
3.4.22.35 complement factor C9 + H2O
-
 Entamoeba histolytica peptides product determination ?
3.4.22.35 fibronectin + H2O
-
 Entamoeba histolytica peptides product determination ?
3.4.22.35 immunoglobulin G + H2O
-
 Entamoeba histolytica peptides derived from immunoglobulin G product determination ?
3.4.22.35 additional information no activity with peptide substrates containing phenylalanine at P2 position instead of arginine Entamoeba histolytica ?
-
 ?
3.4.22.35 protein + H2O enzyme is involved in pathogenic destruction of host tissue by degradation of extracellular matrix proteins Entamoeba histolytica peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.35 More three-dimensional structure modeling Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
3.4.22.35 CP5
-
 Entamoeba histolytica
3.4.22.35 More enzyme is a member of the cysteine proteinase family Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.35 6 8
-
 Entamoeba histolytica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.35 Entamoeba histolytica
-
-
 8.12