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Literature summary extracted from

  • Al-Ajlan, A.; Bailey, G.S.
    Purification and characterization of cationic chymotrypsin from the pancreas of the Arabian camel (Camelus dromedarius) (2000), Mol. Cell. Biochem., 203, 73-78.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.1 Aprotinin
-
 Bos taurus
3.4.21.1 Aprotinin
-
 Camelus dromedarius
3.4.21.1 Lima bean trypsin inhibitor
-
 Bos taurus
3.4.21.1 Lima bean trypsin inhibitor
-
 Camelus dromedarius
3.4.21.1 Soybean trypsin inhibitor
-
 Bos taurus
3.4.21.1 Soybean trypsin inhibitor
-
 Camelus dromedarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.1 0.12
-
 N-benzoyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus
3.4.21.1 0.13
-
 N-benzoyl-L-tyrosine ethyl ester 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 0.14
-
 N-acetyl-L-tryptophan ethyl ester 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 0.19
-
 N-acetyl-L-tryptophan ethyl ester 25°C, pH 7.5 Bos taurus
3.4.21.1 0.57
-
 N-acetyl-L-tyrosine ethyl ester 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 1.12
-
 N-acetyl-L-phenylalanine ethyl ester 25°C, pH 7.5 Bos taurus
3.4.21.1 1.17
-
 N-acetyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus
3.4.21.1 2.58
-
 N-acetyl-L-phenylalanine ethyl ester 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 7.02
-
 N-acetyl-L-leucine methyl ester 25°C, pH 7.5 Bos taurus
3.4.21.1 8.72
-
 N-acetyl-L-leucine methyl ester 25°C, pH 7.5 Camelus dromedarius

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.1 Bos taurus
-
-
-
3.4.21.1 Camelus dromedarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.1 anion exchange column, cation exchange column, affinity F2 Camelus dromedarius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.1 pancreas
-
 Bos taurus
-
3.4.21.1 pancreas
-
 Camelus dromedarius
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.1 78.4
-
 hydrolysisi of N-benzoyl-L-tyrosine ethyl ester Camelus dromedarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.1 N-acetyl-L-leucine methyl ester + H2O 5% of kcat with N-acetyl-L-tyrosine ethyl ester Bos taurus N-acetyl-L-leucine + methanol
-
 ?
3.4.21.1 N-acetyl-L-leucine methyl ester + H2O 5% of kcat with N-acetyl-L-tyrosine ethyl ester Camelus dromedarius N-acetyl-L-leucine + methanol
-
 ?
3.4.21.1 N-acetyl-L-phenylalanine ethyl ester + H2O
-
 Bos taurus N-acetyl-L-phenylalanine + ethanol
-
 ?
3.4.21.1 N-acetyl-L-phenylalanine ethyl ester + H2O
-
 Camelus dromedarius N-acetyl-L-phenylalanine + ethanol
-
 ?
3.4.21.1 N-acetyl-L-tryptophan ethyl ester + H2O
-
 Bos taurus N-acetyl-L-tryptophan + ethanol
-
 ?
3.4.21.1 N-acetyl-L-tryptophan ethyl ester + H2O
-
 Camelus dromedarius N-acetyl-L-tryptophan + ethanol
-
 ?
3.4.21.1 N-acetyl-L-tyrosine ethyl ester + H2O
-
 Bos taurus N-acetyl-L-tyrosine + ethanol
-
 ?
3.4.21.1 N-acetyl-L-tyrosine ethyl ester + H2O
-
 Camelus dromedarius N-acetyl-L-tyrosine + ethanol
-
 ?
3.4.21.1 N-benzoyl-L-tyrosine ethyl ester + H2O
-
 Bos taurus N-benzoyl-L-tyrosine + ethanol
-
 ?
3.4.21.1 N-benzoyl-L-tyrosine ethyl ester + H2O
-
 Camelus dromedarius N-benzoyl-L-tyrosine + ethanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.1 alpha chymotrypsin
-
 Bos taurus
3.4.21.1 cationic chymotrypsin
-
 Camelus dromedarius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.1 1.3
-
 N-benzoyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus
3.4.21.1 4.6
-
 N-acetyl-L-leucine methyl ester 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 6.5
-
 N-acetyl-L-leucine methyl ester 25°C, pH 7.5 Bos taurus
3.4.21.1 25.7
-
 N-acetyl-L-tryptophan ethyl ester 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 40.3
-
 N-acetyl-L-phenylalanine ethyl ester 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 45.9
-
 N-benzoyl-L-tyrosine ethyl ester 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 48.8
-
 N-acetyl-L-tryptophan ethyl ester 25°C, pH 7.5 Bos taurus
3.4.21.1 54.8
-
 N-acetyl-L-phenylalanine ethyl ester 25°C, pH 7.5 Bos taurus
3.4.21.1 70.4
-
 N-benzoyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus
3.4.21.1 82.8
-
 N-acetyl-L-tyrosine ethyl ester 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 119.5
-
 N-acetyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus
3.4.21.1 120
-
 N-acetyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.1 8
-
 hydrolysis of N-benzoyl-L-tyrosine ethyl ester Camelus dromedarius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.1 4.5 9.8 approx. 1% of maximal activity at pH 4.5, approx. 5% of maximal activity at pH 9.8 Camelus dromedarius

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.1 0.0000075
-
 Aprotinin 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 0.000018
-
 Aprotinin 25°C, pH 7.5 Bos taurus
3.4.21.1 0.00013
-
 Lima bean trypsin inhibitor 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 0.00017
-
 Lima bean trypsin inhibitor 25°C, pH 7.5 Bos taurus
3.4.21.1 0.00067
-
 Soybean trypsin inhibitor 25°C, pH 7.5 Camelus dromedarius
3.4.21.1 0.0019
-
 Soybean trypsin inhibitor 25°C, pH 7.5 Bos taurus