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Literature summary extracted from

  • Bussiere, D.E.; Pratt, S.D.; Katz, L.; Severin, J.M.; Holzman, T.; Park, C.H.
    The structure of VanX reveals a novel amino-dipeptidase involved in mediating transposon-based vancomycin resistance (1998), Mol. Cell, 2, 75-84.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.13.22 medicine key drug target in circumventing clinical vancomycin resistance Enterococcus faecium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.13.22 enzyme-D-Ala complex, enzyme-D-Ala-D-Ala complex, and enzyme in complex with phosphonate and phosphinate transition-state analogue inhibitors, complexsitting drops, 10 mg/ml protein, plus equal volume of precipitant solution: 0.25 M ammonium sulfate, 25% w/v polyethylene glycol monomethyl ester 5000, 0.1 M MES, 1 mM ZnCl2, X-ray diffraction at 2.1 A resolution structure determination and analysis Enterococcus faecium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.22 Cu2+
-
 Enterococcus faecium
3.4.13.22 D-3-[(1-aminoethyl)phosphinyl]-D-2-methylpropionic acid phosphinate analogue of the proposed tetrahedral intermediate of the hydrolysis reaction Enterococcus faecium
3.4.13.22 D-3-[(1-aminoethyl)phosphonyl]-D-2-methylpropionic acid phosphonate analogue of the proposed tetrahedral intermediate of the hydrolysis reaction Enterococcus faecium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.22 Zn2+ dependent on Enterococcus faecium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.13.22 D-Ala-D-Ala + H2O Enterococcus faecium
-
 D-Ala + D-Ala
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.22 Enterococcus faecium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.22
-
 Enterococcus faecium

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.13.22 D-Ala-D-Ala + H2O = 2 D-Ala active site structure, substrate binding and mechanism Enterococcus faecium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.22 D-Ala-D-Ala + H2O
-
 Enterococcus faecium D-Ala + D-Ala
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.13.22 More monomer secondary structure Enterococcus faecium

Synonyms

EC Number Synonyms Comment Organism
3.4.13.22 D-alanyl-D-alanine dipeptidase
-
 Enterococcus faecium
3.4.13.22 M15.011 Merops-ID Enterococcus faecium
3.4.13.22 VanX
-
 Enterococcus faecium