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Literature summary extracted from

  • Weber, E.R.; Hanekamp, T.; Thorsness, P.E.
    Biochemical and functional analysis of the YME1 gene product, an ATP and zinc-dependent mitochondrial protease from S. cerevisiae (1996), Mol. Biol. Cell, 7, 307-317.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B19 expression of wild-type enzyme in Escherichia coli strain DH5alpha, expression of deletion mutant in Escherichia coli strain BMH 71-18 mut S Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.B19 additional information construction of inactive mutant by site-directed mutagenesis in the ATP and Zn2+ binding domains, inactivation of the enzyme causes several distinct phenotypes: an increased rate of DNA escape from mitochondria, temperature-sensitive growth on nonfermentable carbon sources, extremely slow growth when mitochondrial DNA is completely absent from the cell, and altered morphology of the mitochondrial compartment Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B19 deoxycholate
-
Saccharomyces cerevisiae
3.4.24.B19 proteinase K
-
Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B19 mitochondrial inner membrane
-
Saccharomyces cerevisiae 5743
-
3.4.24.B19 mitochondrion tightly associated with the inner mitochondrial membrane, oriented with the bulk of the protein facing the matrix Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B19 Zn2+ dependent on, 2 zinc ligands are the conserved His540 and His546 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B19 unassembled gamma subunit of mitochondrial ATP-synthase + H2O Saccharomyces cerevisiae i.e. Atp3p ?
-
?
3.4.24.B19 unassembled subunit II of cytochrome oxidase + H2O Saccharomyces cerevisiae i.e. Cox2p ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B19 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B19 partial Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B19 unassembled gamma subunit of mitochondrial ATP-synthase + H2O i.e. Atp3p Saccharomyces cerevisiae ?
-
?
3.4.24.B19 unassembled subunit II of cytochrome oxidase + H2O i.e. Cox2p Saccharomyces cerevisiae ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B19 AAA protease
-
Saccharomyces cerevisiae
3.4.24.B19 M41.004 Merops-ID Saccharomyces cerevisiae
3.4.24.B19 Yme1p
-
Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.24.B19 ATP required, contains ATP-binding site spanning from residues 316 to 328, GVLLTGPPGTGKT Saccharomyces cerevisiae