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Literature summary extracted from

  • Mathieu, M.A.; Bogyo, M.; Caffrey, C.R.; Choe, Y.; Lee, J.; Chapman, H.; Sajid, M.; Craik, C.S.; McKerrow, J.H.
    Substrate specificity of schistosome versus human legumain determined by P1-P3 peptide libraries (2002), Mol. Biochem. Parasitol., 121, 99-105.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.34 N197C site-directed mutagenesis, the mutation renders the enzyme of Sm32 active Schistosoma mansoni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.34 0.07
-
 acetyl-Thr-Ala-Asn-4-methylcoumarin-7-amide pH 5.5, 25°C Schistosoma mansoni
3.4.22.34 0.08
-
 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide pH 5.5, 25°C Homo sapiens
3.4.22.34 0.09
-
 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide pH 5.5, 25°C Schistosoma mansoni
3.4.22.34 0.122
-
 acetyl-Pro-Thr-Asn-4-methylcoumarin-7-amide pH 5.5, 25°C Homo sapiens
3.4.22.34 0.128
-
 acetyl-Thr-Ala-Asn-4-methylcoumarin-7-amide pH 5.5, 25°C Homo sapiens
3.4.22.34 0.286
-
 acetyl-Pro-Thr-Asn-4-methylcoumarin-7-amide pH 5.5, 25°C Schistosoma mansoni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.34 protein + H2O Homo sapiens
-
 peptides
-
 ?
3.4.22.34 protein + H2O Schistosoma mansoni
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Homo sapiens
-
-
-
3.4.22.34 Schistosoma mansoni
-
 2 recombinant isoforms Sm32 and N197CSm32
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 acetyl-Pro-Thr-Asn-4-methylcoumarin-7-amide + H2O
-
 Homo sapiens acetyl-Pro-Thr-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 acetyl-Pro-Thr-Asn-4-methylcoumarin-7-amide + H2O
-
 Schistosoma mansoni acetyl-Pro-Thr-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 acetyl-Thr-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Homo sapiens acetyl-Thr-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 acetyl-Thr-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Schistosoma mansoni acetyl-Thr-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Homo sapiens benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Schistosoma mansoni benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 cathepsin B proform + H2O cleavage at the sequence Asp-Trp-Asn-Val-Ile-Pro, cleavage site between Asn and Val, between the prodomain and the mature regions of the protein, activation of the substrate, recombinant substrate from Schistosoma mansoni Schistosoma mansoni activated cathepsin B
-
 ?
3.4.22.34 additional information no activity with acetyl-Phe-Tyr-Asn-4-methylcoumarin-7-amide Homo sapiens ?
-
 ?
3.4.22.34 additional information no activity with acetyl-Phe-Tyr-Asn-4-methylcoumarin-7-amide Schistosoma mansoni ?
-
 ?
3.4.22.34 protein + H2O
-
 Homo sapiens peptides
-
 ?
3.4.22.34 protein + H2O
-
 Schistosoma mansoni peptides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.34 Asparaginyl endopeptidase
-
 Homo sapiens
3.4.22.34 Asparaginyl endopeptidase
-
 Schistosoma mansoni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.34 25
-
 assay at Homo sapiens
3.4.22.34 25
-
 assay at Schistosoma mansoni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.34 5.5
-
 assay at Homo sapiens
3.4.22.34 5.5
-
 assay at Schistosoma mansoni