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Literature summary extracted from

  • Lüersen, K.; Muller, S.; Hussein, A.; Liebau, E.; Walter, R.D.
    The gamma-glutamylcysteine synthetase of Onchocerca volvulus (2000), Mol. Biochem. Parasitol., 111, 243-251.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.2.2 pharmacology the enzyme is a potential drug target Onchocerca volvulus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.2 DNA and amino acid sequence determination and analysis, single copy gene, genomic structure Onchocerca volvulus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 cystamine irreversible Onchocerca volvulus
6.3.2.2 L-buthionine-R,S-sulfoximine i.e. BSO, specific, irreversible Onchocerca volvulus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.2 additional information
-
 additional information preliminary steady state kinetics Onchocerca volvulus
6.3.2.2 1.2
-
 L-glutamate pH 8.0, 37°C Onchocerca volvulus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.2 74200
-
 x * 74200, amino acid sequence calculation Onchocerca volvulus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys Onchocerca volvulus first and rate-limiting step in the glutathione biosynthesis, important for maintenance of the intracellular thiol redox status and in detoxification processes ADP + phosphate + gamma-L-Glu-L-Cys
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Onchocerca volvulus Q9NFN6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys
-
 Onchocerca volvulus ADP + phosphate + gamma-L-Glu-L-Cys
-
 r
6.3.2.2 ATP + L-Glu + L-Cys first and rate-limiting step in the glutathione biosynthesis, important for maintenance of the intracellular thiol redox status and in detoxification processes Onchocerca volvulus ADP + phosphate + gamma-L-Glu-L-Cys
-
 r

Subunits

EC Number Subunits Comment Organism
6.3.2.2 ? x * 74200, amino acid sequence calculation Onchocerca volvulus

Synonyms

EC Number Synonyms Comment Organism
6.3.2.2 gamma-GCS
-
 Onchocerca volvulus
6.3.2.2 gamma-Glutamylcysteine synthetase
-
 Onchocerca volvulus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.2 37
-
 assay at Onchocerca volvulus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.2 8
-
 assay at Onchocerca volvulus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.2 ATP
-
 Onchocerca volvulus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.2.2 0.00013
-
 L-buthionine-R,S-sulfoximine pH 8.0, 37°C Onchocerca volvulus
6.3.2.2 0.0039
-
 cystamine pH 8.0, 37°C Onchocerca volvulus