BRENDA - Enzyme Database

Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway

Nakada, Y.; Itoh, Y.; Microbiology 149, 707-714 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.3.12
-
Pseudomonas aeruginosa
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.12
additional information
mutant strain PAO4541
Pseudomonas aeruginosa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.53
Hg2+
79% inhibition at 0.1 mM
Pseudomonas aeruginosa
3.5.1.53
iodoacetamide
88% inhibition at 1 mM
Pseudomonas aeruginosa
3.5.1.53
additional information
not inhibitory at 1 mM: arginine, ornithine, EDTA, putrescine, spermidine, spermine
Pseudomonas aeruginosa
3.5.1.53
p-chloromercuribenzoate
94% inhibition at 0.1 mM
Pseudomonas aeruginosa
3.5.3.12
cationic mercury
enzyme 90% inhibited at 0.1 mM
Pseudomonas aeruginosa
3.5.3.12
iodoacetoamide
enzyme 95% inhibited at 0.1 mM
Pseudomonas aeruginosa
3.5.3.12
p-chloromercuribenzoate
enzyme 12% inhibited at 0.1 mM
Pseudomonas aeruginosa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.53
0.5
-
N-Carbamoylputrescine
-
Pseudomonas aeruginosa
3.5.3.12
0.6
-
agmatine
-
Pseudomonas aeruginosa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.53
33000
-
6 * 33000, SDS-PAGE
Pseudomonas aeruginosa
3.5.1.53
230000
-
gel filtration
Pseudomonas aeruginosa
3.5.3.12
43000
-
2 * 43000, SDS-PAGE
Pseudomonas aeruginosa
3.5.3.12
85000
-
cross linkage
Pseudomonas aeruginosa
3.5.3.12
89000
-
gel filtration
Pseudomonas aeruginosa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.53
Pseudomonas aeruginosa
-
-
-
3.5.3.12
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.12
strain PAO4495, 21fold purified to near homogeneity, gel filtration
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.53
N-carbamoylcadaverine + H2O
poor substrate
653166
Pseudomonas aeruginosa
cadaverine + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
3.5.1.53
N-carbamoyldiaminopropane + H2O
poor substrate
653166
Pseudomonas aeruginosa
diaminopropane + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
3.5.1.53
N-carbamoylputrescine + H2O
-
653166
Pseudomonas aeruginosa
putrescine + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
3.5.3.12
agmatine + H2O
sole substrate for AguA
653166
Pseudomonas aeruginosa
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
additional information
no activity with arginine, creatine, creatinine, guanidinoacetate, 3-guanidinopropionate and 4-guanidinobutytrate as substrates
653166
Pseudomonas aeruginosa
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.53
homohexamer
6 * 33000, SDS-PAGE
Pseudomonas aeruginosa
3.5.3.12
homodimer
2 * 43000, SDS-PAGE
Pseudomonas aeruginosa
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.53
40
-
-
Pseudomonas aeruginosa
3.5.3.12
45
-
-
Pseudomonas aeruginosa
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.12
8
-
agmatine
-
Pseudomonas aeruginosa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.53
8
-
-
Pseudomonas aeruginosa
3.5.3.12
8
-
in Tris-HCl buffer
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.12
-
Pseudomonas aeruginosa
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.12
additional information
mutant strain PAO4541
Pseudomonas aeruginosa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.53
Hg2+
79% inhibition at 0.1 mM
Pseudomonas aeruginosa
3.5.1.53
iodoacetamide
88% inhibition at 1 mM
Pseudomonas aeruginosa
3.5.1.53
additional information
not inhibitory at 1 mM: arginine, ornithine, EDTA, putrescine, spermidine, spermine
Pseudomonas aeruginosa
3.5.1.53
p-chloromercuribenzoate
94% inhibition at 0.1 mM
Pseudomonas aeruginosa
3.5.3.12
cationic mercury
enzyme 90% inhibited at 0.1 mM
Pseudomonas aeruginosa
3.5.3.12
iodoacetoamide
enzyme 95% inhibited at 0.1 mM
Pseudomonas aeruginosa
3.5.3.12
p-chloromercuribenzoate
enzyme 12% inhibited at 0.1 mM
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.53
0.5
-
N-Carbamoylputrescine
-
Pseudomonas aeruginosa
3.5.3.12
0.6
-
agmatine
-
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.53
33000
-
6 * 33000, SDS-PAGE
Pseudomonas aeruginosa
3.5.1.53
230000
-
gel filtration
Pseudomonas aeruginosa
3.5.3.12
43000
-
2 * 43000, SDS-PAGE
Pseudomonas aeruginosa
3.5.3.12
85000
-
cross linkage
Pseudomonas aeruginosa
3.5.3.12
89000
-
gel filtration
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.12
strain PAO4495, 21fold purified to near homogeneity, gel filtration
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.53
N-carbamoylcadaverine + H2O
poor substrate
653166
Pseudomonas aeruginosa
cadaverine + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
3.5.1.53
N-carbamoyldiaminopropane + H2O
poor substrate
653166
Pseudomonas aeruginosa
diaminopropane + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
3.5.1.53
N-carbamoylputrescine + H2O
-
653166
Pseudomonas aeruginosa
putrescine + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
3.5.3.12
agmatine + H2O
sole substrate for AguA
653166
Pseudomonas aeruginosa
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
additional information
no activity with arginine, creatine, creatinine, guanidinoacetate, 3-guanidinopropionate and 4-guanidinobutytrate as substrates
653166
Pseudomonas aeruginosa
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.53
homohexamer
6 * 33000, SDS-PAGE
Pseudomonas aeruginosa
3.5.3.12
homodimer
2 * 43000, SDS-PAGE
Pseudomonas aeruginosa
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.53
40
-
-
Pseudomonas aeruginosa
3.5.3.12
45
-
-
Pseudomonas aeruginosa
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.12
8
-
agmatine
-
Pseudomonas aeruginosa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.53
8
-
-
Pseudomonas aeruginosa
3.5.3.12
8
-
in Tris-HCl buffer
Pseudomonas aeruginosa