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Literature summary extracted from

  • Ramirez-Coronel, M.A.; Viniegra-Gonzalez, G.; Darvill, A.; Augur, C.
    A novel tannase from Aspergillus niger with beta-glucosidase activity (2003), Microbiology, 149, 2941-2946.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.20 extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 90000
-
 x * 90000, SDS-PAGE, x * 180000, SDS-PAGE Aspergillus niger
3.1.1.20 180000
-
 x * 90000, SDS-PAGE, x * 180000, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus niger
-
 IRDUAM collection strain No. Aa20
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 to homogeneity from culture supernatant Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 additional information
-
-
 Aspergillus niger
3.1.1.20 0.18
-
 purified enzyme, substrate catechin gallate Aspergillus niger
3.1.1.20 0.43
-
 purified enzyme, substrate epicatechin gallate Aspergillus niger
3.1.1.20 0.64
-
 purified enzyme, substrate epigallocatechin gallate Aspergillus niger
3.1.1.20 0.84
-
 purified enzyme, substrate tannic acid Aspergillus niger
3.1.1.20 0.87
-
 purified enzyme, substrate gallic acid methyl ester Aspergillus niger
3.1.1.20 1.13
-
 purified enzyme, substrate gallocatechin gallate Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 catechin gallate + H2O
-
 Aspergillus niger gallic acid + catechin
-
 ?
3.1.1.20 cellobiose + H2O beta-glucosidase activity of the enzyme, only observed in absence of tannic acid Aspergillus niger 2 D-glucose
-
 ?
3.1.1.20 epicatechin gallate + H2O
-
 Aspergillus niger gallic acid + epicatechin
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Aspergillus niger gallic acid + epigallocatechin
-
 ?
3.1.1.20 gallic acid methyl ester + H2O
-
 Aspergillus niger gallic acid + methanol
-
 ?
3.1.1.20 gallocatechin gallate + H2O best substrate Aspergillus niger gallic acid + gallocatechin
-
 ?
3.1.1.20 additional information the enzyme removes gallic acid non-specifically from both condensed and hydrolysable tannins Aspergillus niger ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus niger 10 gallate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 ? x * 90000, SDS-PAGE, x * 180000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 60 70
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 30 90 markedly reduced activity above 70°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 6
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.20 3.5 7
-
 Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.20 Aspergillus niger isoelectric focusing
-
 3.8