BRENDA - Enzyme Database

Physiological role of S-formylglutathione hydrolase in C(1) metabolism of the methylotrophic yeast Candida boidinii

Yurimoto, H.; Lee, B.; Yano, T.; Sakai, Y.; Kato, N.; Microbiology 149, 1971-1979 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.2.12
gene fgh1, DNA and amino acid sequence determination and analysis, expression in DH5alpha, expression as GFP-fusion protein in Candida boidinii
Candida boidinii
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.2.12
additional information
fgh1 gene disruption impairs the ability to grow on methanol and reduces the growth rate on methylamine and choline as sole nitrogen source, while growth on glucose or glycerol is not affected
Candida boidinii
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.12
cytosol
detection of recombinant GFP-fusion protein
Candida boidinii
5829
-
3.1.2.12
peroxisome
detection of recombinant GFP-fusion protein
Candida boidinii
5777
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.12
S-formylglutathione + H2O
Candida boidinii
enzyme is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source, regulation of gene expression
glutathione + formate
-
Candida boidinii
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.12
Candida boidinii
-
methylotrophic yeast, gene fgh1, strain S2
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
S-formylglutathione + H2O
-
653164
Candida boidinii
glutathione + formate
-
653164
Candida boidinii
?
3.1.2.12
S-formylglutathione + H2O
enzyme is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source, regulation of gene expression
653164
Candida boidinii
glutathione + formate
-
653164
Candida boidinii
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.12
30
-
assay at
Candida boidinii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
6
-
assay at
Candida boidinii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.12
gene fgh1, DNA and amino acid sequence determination and analysis, expression in DH5alpha, expression as GFP-fusion protein in Candida boidinii
Candida boidinii
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.2.12
additional information
fgh1 gene disruption impairs the ability to grow on methanol and reduces the growth rate on methylamine and choline as sole nitrogen source, while growth on glucose or glycerol is not affected
Candida boidinii
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.12
cytosol
detection of recombinant GFP-fusion protein
Candida boidinii
5829
-
3.1.2.12
peroxisome
detection of recombinant GFP-fusion protein
Candida boidinii
5777
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.12
S-formylglutathione + H2O
Candida boidinii
enzyme is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source, regulation of gene expression
glutathione + formate
-
Candida boidinii
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
S-formylglutathione + H2O
-
653164
Candida boidinii
glutathione + formate
-
653164
Candida boidinii
?
3.1.2.12
S-formylglutathione + H2O
enzyme is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source, regulation of gene expression
653164
Candida boidinii
glutathione + formate
-
653164
Candida boidinii
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.12
30
-
assay at
Candida boidinii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
6
-
assay at
Candida boidinii