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Literature summary extracted from

  • Yurimoto, H.; Lee, B.; Yano, T.; Sakai, Y.; Kato, N.
    Physiological role of S-formylglutathione hydrolase in C(1) metabolism of the methylotrophic yeast Candida boidinii (2003), Microbiology, 149, 1971-1979.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.12 gene fgh1, DNA and amino acid sequence determination and analysis, expression in DH5alpha, expression as GFP-fusion protein in Candida boidinii [Candida] boidinii

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.12 additional information fgh1 gene disruption impairs the ability to grow on methanol and reduces the growth rate on methylamine and choline as sole nitrogen source, while growth on glucose or glycerol is not affected [Candida] boidinii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.12 cytosol detection of recombinant GFP-fusion protein [Candida] boidinii 5829
-
3.1.2.12 peroxisome detection of recombinant GFP-fusion protein [Candida] boidinii 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.12 S-formylglutathione + H2O [Candida] boidinii enzyme is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source, regulation of gene expression glutathione + formate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.12 [Candida] boidinii
-
 methylotrophic yeast, gene fgh1, strain S2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.12 S-formylglutathione + H2O
-
 [Candida] boidinii glutathione + formate
-
 ?
3.1.2.12 S-formylglutathione + H2O enzyme is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source, regulation of gene expression [Candida] boidinii glutathione + formate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.12 FGH
-
 [Candida] boidinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.12 30
-
 assay at [Candida] boidinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.12 6
-
 assay at [Candida] boidinii