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Literature summary extracted from

  • Blank, L.; Green, J.; Guest, J.R.
    AcnC of Escherichia coli is a 2-methylcitrate dehydratase (PrpD) that can use citrate and isocitrate as substrates (2002), Microbiology, 148, 133-146.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.79 propionate induces the enzyme expression from acnC and prpD Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.79 DNA and amino acid sequence determination and analysis, genomic organization, expression of AcnC in AcnABnull strain, overexpression of PrpD in AcnABnull strain Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.79 Fe2+ enzyme possesses 1 unstable iron-sulfur center per monomer, required for activity, can be reconstituted by Fe2+ under reducing conditions Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.79 54000
-
 gel filtration and native PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate Escherichia coli metabolic function (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.79 Escherichia coli
-
 K12, several strains, gene prpD of the propionate catabolism operon prpRBCDE, the enzyme is identical with aconitase AcnC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.79 AcnC partially 0.25fold from AcnABnull strain, PrpD 14.3fold from overexpression in AcnABnull strain Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.79 0.005
-
 partially purified AcnC Escherichia coli
4.2.1.79 0.8
-
 purified PrpD Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.79 (S)-malate
-
 Escherichia coli ?
-
 ?
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate, best substrate Escherichia coli (Z)-But-2-ene-1,2,3-tricarboxylate + H2O i.e. 2-methyl-cis-aconitate ?
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate metabolic function Escherichia coli (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
 ?
4.2.1.79 citrate
-
 Escherichia coli ?
-
 ?
4.2.1.79 D-malate
-
 Escherichia coli ?
-
 ?
4.2.1.79 D-tartrate
-
 Escherichia coli ?
-
 ?
4.2.1.79 DL-citramalate
-
 Escherichia coli ?
-
 ?
4.2.1.79 DL-isocitrate
-
 Escherichia coli ?
-
 ?
4.2.1.79 L-tartrate
-
 Escherichia coli ?
-
 ?
4.2.1.79 meso-tartrate
-
 Escherichia coli ?
-
 ?
4.2.1.79 additional information substrate specificity, no activity with cis-aconitate and related substances containing double bonds, overview Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.79 monomer 1 * 54000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.2.1.79 PrpD
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.79 37
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.79 50
-
 half-life is 30 min for both AcnC and PrpD Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.79 7 8 for AcnC and PrpD Escherichia coli