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Literature summary extracted from

  • Virtanen, A.; Martinez, J.; Ren, Y.G.
    Purification of poly(A)-specific ribonuclease (2001), Methods Enzymol., 342, 303-309.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.13.4 K+ required, optimal at about 100 mM Homo sapiens
3.1.13.4 K+ required, optimal at about 100 mM Bos taurus
3.1.13.4 K+ required, optimal at about 100 mM Xenopus sp.
3.1.13.4 Mg2+ required, optimal at about 1 mM, preferred kind of divalent cation Homo sapiens
3.1.13.4 Mg2+ required, optimal at about 1 mM, preferred kind of divalent cation Bos taurus
3.1.13.4 Mg2+ required, optimal at about 1 mM, preferred kind of divalent cation Xenopus sp.
3.1.13.4 Na+ may substitute for K+, but less effective Homo sapiens
3.1.13.4 Na+ may substitute for K+, but less effective Bos taurus
3.1.13.4 Na+ may substitute for K+, but less effective Xenopus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.4 Bos taurus
-
-
-
3.1.13.4 Homo sapiens
-
-
-
3.1.13.4 Xenopus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.13.4 overview on conditions Homo sapiens
3.1.13.4 overview on conditions Bos taurus
3.1.13.4 overview on conditions Xenopus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.13.4 HeLa cell
-
 Homo sapiens
-
3.1.13.4 oocyte
-
 Xenopus sp.
-
3.1.13.4 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.13.4 additional information
-
 overview on assay methods and conditions Homo sapiens
3.1.13.4 additional information
-
 overview on assay methods and conditions Bos taurus
3.1.13.4 additional information
-
 overview on assay methods and conditions Xenopus sp.

Storage Stability

EC Number Storage Stability Organism
3.1.13.4 -70°C, stable for several months Homo sapiens
3.1.13.4 -70°C, stable for several months Bos taurus
3.1.13.4 -70°C, stable for several months Xenopus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.4 additional information enzyme is 5-6 times more active if the substrate is m7G(5’)ppp(5’)G capped Homo sapiens ?
-
 ?
3.1.13.4 additional information enzyme is 5-6 times more active if the substrate is m7G(5’)ppp(5’)G capped Bos taurus ?
-
 ?
3.1.13.4 additional information enzyme is 5-6 times more active if the substrate is m7G(5’)ppp(5’)G capped Xenopus sp. ?
-
 ?
3.1.13.4 poly(A)-mRNA + H2O
-
 Homo sapiens 5'-AMP
-
 ?
3.1.13.4 poly(A)-mRNA + H2O
-
 Bos taurus 5'-AMP
-
 ?
3.1.13.4 poly(A)-mRNA + H2O
-
 Xenopus sp. 5'-AMP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.13.4 7
-
-
 Homo sapiens
3.1.13.4 7
-
-
 Bos taurus
3.1.13.4 7
-
-
 Xenopus sp.