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Literature summary extracted from

  • Wurtele, E.S.; Nikolau, B.J.
    Characterization of 3-methylcrotonyl-CoA carboxylase from plants (2000), Methods Enzymol., 324, 280-292.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.4.1.4 MCCase extracted with 0.1 M Tris-HCl pH 8.0 is relatively unstable, enzyme is completely inactive 2-3 days after extraction, changing of pH or addition of bovine serum albumin fails to stabilize the enzyme Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.4 acetoacetyl-CoA
-
 Daucus carota
6.4.1.4 acetoacetyl-CoA
-
 Glycine max
6.4.1.4 acetoacetyl-CoA
-
 Hordeum vulgare
6.4.1.4 acetoacetyl-CoA
-
 Pisum sativum
6.4.1.4 acetoacetyl-CoA
-
 Solanum tuberosum
6.4.1.4 acetoacetyl-CoA
-
 Zea mays
6.4.1.4 Li+
-
 Daucus carota
6.4.1.4 Li+
-
 Glycine max
6.4.1.4 Li+
-
 Hordeum vulgare
6.4.1.4 Li+
-
 Pisum sativum
6.4.1.4 Li+
-
 Solanum tuberosum
6.4.1.4 Li+
-
 Zea mays
6.4.1.4 Na+
-
 Daucus carota
6.4.1.4 Na+
-
 Glycine max
6.4.1.4 Na+
-
 Hordeum vulgare
6.4.1.4 Na+
-
 Pisum sativum
6.4.1.4 Na+
-
 Solanum tuberosum
6.4.1.4 Na+
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.4 0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Hordeum vulgare
6.4.1.4 0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Pisum sativum
6.4.1.4 0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Zea mays
6.4.1.4 0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Solanum tuberosum
6.4.1.4 0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Glycine max
6.4.1.4 0.01 0.05 3-methylcrotonoyl-CoA pH 8.0, 37°C Daucus carota
6.4.1.4 0.02
-
 ATP pH 8.0, 37°C Hordeum vulgare
6.4.1.4 0.02
-
 ATP pH 8.0, 37°C Pisum sativum
6.4.1.4 0.02
-
 ATP pH 8.0, 37°C Zea mays
6.4.1.4 0.02
-
 ATP pH 8.0, 37°C Solanum tuberosum
6.4.1.4 0.02
-
 ATP pH 8.0, 37°C Glycine max
6.4.1.4 0.02
-
 ATP pH 8.0, 37°C Daucus carota
6.4.1.4 0.82
-
 3-methylcrotonoyl-CoA pH 8.0, 37°C Hordeum vulgare
6.4.1.4 0.82
-
 3-methylcrotonoyl-CoA pH 8.0, 37°C Pisum sativum
6.4.1.4 0.82
-
 3-methylcrotonoyl-CoA pH 8.0, 37°C Zea mays
6.4.1.4 0.82
-
 3-methylcrotonoyl-CoA pH 8.0, 37°C Solanum tuberosum
6.4.1.4 0.82
-
 3-methylcrotonoyl-CoA pH 8.0, 37°C Glycine max
6.4.1.4 0.82
-
 3-methylcrotonoyl-CoA pH 8.0, 37°C Daucus carota

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.4.1.4 mitochondrion
-
 Daucus carota 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.4 Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Hordeum vulgare
6.4.1.4 Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Pisum sativum
6.4.1.4 Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Zea mays
6.4.1.4 Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Solanum tuberosum
6.4.1.4 Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Glycine max
6.4.1.4 Co2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Daucus carota
6.4.1.4 Cs+ activator Hordeum vulgare
6.4.1.4 Cs+ activator Pisum sativum
6.4.1.4 Cs+ activator Zea mays
6.4.1.4 Cs+ activator Solanum tuberosum
6.4.1.4 Cs+ activator Glycine max
6.4.1.4 Cs+ activator Daucus carota
6.4.1.4 K+ activator Hordeum vulgare
6.4.1.4 K+ activator Pisum sativum
6.4.1.4 K+ activator Zea mays
6.4.1.4 K+ activator Solanum tuberosum
6.4.1.4 K+ activator Glycine max
6.4.1.4 K+ activator Daucus carota
6.4.1.4 Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Hordeum vulgare
6.4.1.4 Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Pisum sativum
6.4.1.4 Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Zea mays
6.4.1.4 Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Solanum tuberosum
6.4.1.4 Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Glycine max
6.4.1.4 Mg2+ requires free Mg2+ ions for activation, in excess of thatrequired to complex ATP Daucus carota
6.4.1.4 Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Hordeum vulgare
6.4.1.4 Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Pisum sativum
6.4.1.4 Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Zea mays
6.4.1.4 Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Solanum tuberosum
6.4.1.4 Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Glycine max
6.4.1.4 Mn2+ requirement for free Mg2+ ions can be partially replaced by Mn2+ or Co2+, but not by Zn2+ Daucus carota
6.4.1.4 NH4+ activator Hordeum vulgare
6.4.1.4 NH4+ activator Pisum sativum
6.4.1.4 NH4+ activator Zea mays
6.4.1.4 NH4+ activator Solanum tuberosum
6.4.1.4 NH4+ activator Glycine max
6.4.1.4 NH4+ activator Daucus carota
6.4.1.4 Rb+ activator Hordeum vulgare
6.4.1.4 Rb+ activator Pisum sativum
6.4.1.4 Rb+ activator Zea mays
6.4.1.4 Rb+ activator Solanum tuberosum
6.4.1.4 Rb+ activator Glycine max
6.4.1.4 Rb+ activator Daucus carota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.4 60000
-
 4 * 80000 + 4 * 60000, SDS-PAGE Pisum sativum
6.4.1.4 60000
-
 4 * 80000 + 4 * 60000, SDS-PAGE Solanum tuberosum
6.4.1.4 60000
-
 6 * 800000 + 6 * 60000 Zea mays
6.4.1.4 60000
-
 6 * 800000 + 6 * 60000 Glycine max
6.4.1.4 60000
-
 6 * 800000 + 6 * 60000 Daucus carota
6.4.1.4 60000
-
 6 * 800000 + 6 * 60000 Solanum lycopersicum
6.4.1.4 80000
-
 4 * 80000 + 4 * 60000, SDS-PAGE Pisum sativum
6.4.1.4 80000
-
 4 * 80000 + 4 * 60000, SDS-PAGE Solanum tuberosum
6.4.1.4 500000
-
 gel filtration Pisum sativum
6.4.1.4 500000
-
 gel filtration Solanum tuberosum
6.4.1.4 800000
-
 6 * 800000 + 6 * 60000 Zea mays
6.4.1.4 800000
-
 6 * 800000 + 6 * 60000 Glycine max
6.4.1.4 800000
-
 6 * 800000 + 6 * 60000 Daucus carota
6.4.1.4 800000
-
 6 * 800000 + 6 * 60000 Solanum lycopersicum
6.4.1.4 800000 900000 nondenaturing PAGE Zea mays
6.4.1.4 800000 900000 nondenaturing PAGE Glycine max
6.4.1.4 800000 900000 nondenaturing PAGE Daucus carota
6.4.1.4 800000 900000 nondenaturing PAGE Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- Hordeum vulgare in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- Pisum sativum in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- Zea mays in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- Solanum tuberosum in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- Glycine max in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- Daucus carota in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- Solanum lycopersicum in addition to leucine catabolism required for the operation of the mevalonate shunt ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.4 Daucus carota
-
 carrot
-
6.4.1.4 Glycine max
-
 soybean, cv. Corsoy 79
-
6.4.1.4 Hordeum vulgare
-
 barley
-
6.4.1.4 Pisum sativum
-
 pea
-
6.4.1.4 Solanum lycopersicum
-
 tomato
-
6.4.1.4 Solanum tuberosum
-
 potato
-
6.4.1.4 Zea mays
-
 maize
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.4
-
 Hordeum vulgare
6.4.1.4
-
 Pisum sativum
6.4.1.4
-
 Zea mays
6.4.1.4
-
 Solanum tuberosum
6.4.1.4
-
 Glycine max
6.4.1.4
-
 Daucus carota
6.4.1.4
-
 Solanum lycopersicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.4 0.2062
-
-
 Zea mays
6.4.1.4 0.3367
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
6.4.1.4 -20°C, purified rnzyme can be stored up to 6 months with minimal loss of activity Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3-
-
 Hordeum vulgare ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3-
-
 Pisum sativum ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3-
-
 Zea mays ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3-
-
 Solanum tuberosum ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3-
-
 Glycine max ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3-
-
 Daucus carota ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3-
-
 Solanum lycopersicum ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Hordeum vulgare ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Pisum sativum ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Zea mays ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Solanum tuberosum ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Glycine max ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Daucus carota ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- in addition to leucine catabolism required for the operation of the mevalonate shunt Solanum lycopersicum ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
6.4.1.4 ATP + crotonyl-CoA + HCO3- much poorer substrate Hordeum vulgare ADP + phosphate + glutaconyl-CoA
-
 ir
6.4.1.4 ATP + crotonyl-CoA + HCO3- much poorer substrate Pisum sativum ADP + phosphate + glutaconyl-CoA
-
 ir
6.4.1.4 ATP + crotonyl-CoA + HCO3- much poorer substrate Zea mays ADP + phosphate + glutaconyl-CoA
-
 ir
6.4.1.4 ATP + crotonyl-CoA + HCO3- much poorer substrate Solanum tuberosum ADP + phosphate + glutaconyl-CoA
-
 ir
6.4.1.4 ATP + crotonyl-CoA + HCO3- much poorer substrate Glycine max ADP + phosphate + glutaconyl-CoA
-
 ir
6.4.1.4 ATP + crotonyl-CoA + HCO3- much poorer substrate Daucus carota ADP + phosphate + glutaconyl-CoA
-
 ir
6.4.1.4 additional information acetoacetyl-CoA is no substrate Hordeum vulgare ?
-
 ?
6.4.1.4 additional information acetoacetyl-CoA is no substrate Pisum sativum ?
-
 ?
6.4.1.4 additional information acetoacetyl-CoA is no substrate Zea mays ?
-
 ?
6.4.1.4 additional information acetoacetyl-CoA is no substrate Solanum tuberosum ?
-
 ?
6.4.1.4 additional information acetoacetyl-CoA is no substrate Glycine max ?
-
 ?
6.4.1.4 additional information acetoacetyl-CoA is no substrate Daucus carota ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.4 dodecamer 6 * 800000 + 6 * 60000 Zea mays
6.4.1.4 dodecamer 6 * 800000 + 6 * 60000 Glycine max
6.4.1.4 dodecamer 6 * 800000 + 6 * 60000 Daucus carota
6.4.1.4 dodecamer 6 * 800000 + 6 * 60000 Solanum lycopersicum
6.4.1.4 octamer 4 * 80000 + 4 * 60000, SDS-PAGE Pisum sativum
6.4.1.4 octamer 4 * 80000 + 4 * 60000, SDS-PAGE Solanum tuberosum

Synonyms

EC Number Synonyms Comment Organism
6.4.1.4 3-methylcrotonyl-CoA carboxylase
-
 Hordeum vulgare
6.4.1.4 3-methylcrotonyl-CoA carboxylase
-
 Pisum sativum
6.4.1.4 3-methylcrotonyl-CoA carboxylase
-
 Zea mays
6.4.1.4 3-methylcrotonyl-CoA carboxylase
-
 Solanum tuberosum
6.4.1.4 3-methylcrotonyl-CoA carboxylase
-
 Glycine max
6.4.1.4 3-methylcrotonyl-CoA carboxylase
-
 Daucus carota
6.4.1.4 3-methylcrotonyl-CoA carboxylase
-
 Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.4 8 8.5
-
 Hordeum vulgare
6.4.1.4 8 8.5
-
 Pisum sativum
6.4.1.4 8 8.5
-
 Zea mays
6.4.1.4 8 8.5
-
 Solanum tuberosum
6.4.1.4 8 8.5
-
 Glycine max
6.4.1.4 8 8.5
-
 Daucus carota