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Literature summary extracted from

  • Shimomura, Y.; Murakami, T.; Nakai, N.; Huang, B.; Hawes, J.W.; Harris, R.A.
    3-Hydroxyisobutyryl-CoA hydrolase (2000), Methods Enzymol., 324, 229-240.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.4 DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.4 acetoacetyl-CoA competitive inhibition Homo sapiens
3.1.2.4 acetyl-CoA competitive inhibition Homo sapiens
3.1.2.4 DL-3-hydroxybutyryl-CoA competitive inhibition Homo sapiens
3.1.2.4 DL-methylmalonyl-CoA competitive inhibition Homo sapiens
3.1.2.4 isobutyryl-CoA competitive inhibition Homo sapiens
3.1.2.4 malonyl-CoA competitive inhibition Homo sapiens
3.1.2.4 n-valeryl-CoA competitive inhibition Homo sapiens
3.1.2.4 propionyl-CoA competitive inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.4 0.006
-
(S)-3-hydroxy-2-methylpropanoyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
3.1.2.4 0.025
-
3-hydroxypropionyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.4 mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.4 36000
-
gel filtration Rattus norvegicus
3.1.2.4 38000
-
1 * 39398, amino acid sequence calculation, 1 * 38000, recombinant enzyme, SDS-PAGE Homo sapiens
3.1.2.4 39398
-
1 * 39398, amino acid sequence calculation, 1 * 38000, recombinant enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.4 3-hydroxy-2-methylpropanoyl-CoA + H2O Homo sapiens
-
CoA + 3-hydroxy-2-methylpropanoate
-
?
3.1.2.4 3-hydroxy-2-methylpropanoyl-CoA + H2O Rattus norvegicus substrate is an intermediate in the pathway of valine catabolism, which is unique in that it produces a radily diffusible monocarboxylic acid 3-hydroxy-2-methylpropanoate + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.4 Homo sapiens
-
-
-
3.1.2.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.4 from rat liver, 7200fold, to homogeneity Rattus norvegicus
3.1.2.4 recombinant enzyme from Escherichia coli, removal of His-tag with thrombin Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.4 brain
-
Rattus norvegicus
-
3.1.2.4 heart
-
Rattus norvegicus
-
3.1.2.4 kidney
-
Rattus norvegicus
-
3.1.2.4 liver
-
Homo sapiens
-
3.1.2.4 liver
-
Rattus norvegicus
-
3.1.2.4 muscle
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.4 244
-
purified recombinant enzyme, substrate 3-hydroxypropionyl-CoA Homo sapiens
3.1.2.4 427
-
purified enzyme Rattus norvegicus
3.1.2.4 430
-
purified recombinant enzyme, substrate (S)-3-hydroxy-2-methylpropanoyl-CoA Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.4 (S)-3-hydroxy-2-methylpropanoyl-CoA + H2O
-
Homo sapiens CoA + (S)-3-hydroxy-2-methylpropanoate
-
?
3.1.2.4 3-hydroxy-2-methylbutyryl-CoA + H2O low activity Homo sapiens CoA + 3-hydroxy-2-methylbutyrate
-
?
3.1.2.4 3-hydroxy-2-methylpropanoyl-CoA + H2O
-
Homo sapiens CoA + 3-hydroxy-2-methylpropanoate
-
?
3.1.2.4 3-hydroxy-2-methylpropanoyl-CoA + H2O
-
Rattus norvegicus CoA + 3-hydroxy-2-methylpropanoate
-
?
3.1.2.4 3-hydroxy-2-methylpropanoyl-CoA + H2O substrate is an intermediate in the pathway of valine catabolism, which is unique in that it produces a radily diffusible monocarboxylic acid Rattus norvegicus 3-hydroxy-2-methylpropanoate + CoA
-
?
3.1.2.4 3-hydroxypropanoyl-CoA + H2O
-
Homo sapiens CoA + 3-hydroxypropanoate
-
?
3.1.2.4 DL-3-hydroxybutyryl-CoA + H2O low activity Homo sapiens CoA + DL-3-hydroxybutyrate
-
?
3.1.2.4 L-3-hydroxybutyryl-CoA + H2O low activity Homo sapiens CoA + L-3-hydroxybutyrate
-
?
3.1.2.4 additional information substrate specificity, overview, acetoacetyl-CoA, DL-methylmalonyl-CoA, isobutyryl-CoA, malonyl-CoA, acetyl-CoA, propionyl-CoA, and n-valeryl-CoA are poor substrates Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.2.4 monomer 1 * 36000, SDS-PAGE Rattus norvegicus
3.1.2.4 monomer 1 * 39398, amino acid sequence calculation, 1 * 38000, recombinant enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.4 30
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.4 8
-
-
Homo sapiens
3.1.2.4 8
-
assay at Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.4 5.1 10.3 at least Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.4 0.12
-
acetoacetyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
3.1.2.4 0.14
-
DL-3-hydroxybutyryl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
3.1.2.4 0.72
-
n-valeryl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
3.1.2.4 0.83
-
propionyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
3.1.2.4 1.19
-
isobutyryl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
3.1.2.4 1.6
-
malonyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
3.1.2.4 2.26
-
DL-methylmalonyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
3.1.2.4 2.61
-
acetyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens