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Literature summary extracted from

  • Lansmann, S.; Bartelsen, O.; Sandhoff, K.
    Purification and characterization of recombinant human acid sphingomyelinase expressed in insect Sf21 cells (2000), Methods Enzymol., 311, 149-156.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 expression in Sf9 insect cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.032
-
sphingomyelin 37°C, pH 4.8 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 lysosome
-
Homo sapiens 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 72000
-
native PAGE, recombinant haSMase Homo sapiens
3.1.4.12 75000
-
native PAGE, placental haSMase Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Homo sapiens
-
N-acylsphingosine + choline phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.12 glycoprotein molecular mass reduction of approx. 8-9 kDA upon deglycosylation Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 recombinant haSMase, ammonium sulfate, Con A-Sepharose, Octyl-Sepharose, Matrex Gel Red A, DEAE-650 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 7.33
-
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
Homo sapiens N-acylsphingosine + choline phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 acid sphingomyelinase
-
Homo sapiens
3.1.4.12 haSMase
-
Homo sapiens