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Literature summary extracted from

  • Iwamoto, K.; Kawanobe, H.; Shiraiwa, Y.; Ikawa, T.
    Purification and characterization of mannitol-1-phosphatase in the red alga Caloglossa continua (Ceramiales, Rhodophyta) (2001), Mar. Biotechnol., 3, 493-500.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.22 Ca2+ 1 mM, 46% inhibition Caloglossa continua
3.1.3.22 mannitol inhibition of 50% or more Caloglossa continua
3.1.3.22 MnCl2
-
 Caloglossa continua
3.1.3.22 NaCl 250 mM, about 40% inhibition, about 60% inhibition at 500 mM Caloglossa continua
3.1.3.22 NaF 1 mM, 91% inhibition Caloglossa continua
3.1.3.22 NEM 1 mM, complete inhibition, 79% inhibition at 0.1 mM Caloglossa continua
3.1.3.22 p-hydroxymercuribenzoate 0.001 mM, complete inhibition Caloglossa continua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.22 0.41
-
 D-mannitol 1-phosphate
-
 Caloglossa continua

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.22 Mg2+ maximal activation at 1 mM, 25% of the maximum is obtained at 0.1 mM MgCl2 Caloglossa continua

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.22 28500
-
 gel filtration Caloglossa continua
3.1.3.22 30200
-
 1 * 30200, SDS-PAGE Caloglossa continua

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.22 D-mannitol 1-phosphate + H2O Caloglossa continua final step of mannitol biosynthesis D-mannitol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.22 Caloglossa continua
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.22
-
 Caloglossa continua

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.22 62.5
-
-
 Caloglossa continua

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.22 D-fructose 6-phosphate + H2O 1.8% of the activity with D-mannitol 1-phosphate Caloglossa continua D-fructose + phosphate
-
 ?
3.1.3.22 D-mannitol 1-phosphate + H2O
-
 Caloglossa continua D-mannitol + phosphate
-
 ?
3.1.3.22 D-mannitol 1-phosphate + H2O final step of mannitol biosynthesis Caloglossa continua D-mannitol + phosphate
-
 ?
3.1.3.22 sorbitol 1-phosphate + H2O 2.3% of the activity with D-mannitol 1-phosphate Caloglossa continua sorbose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.22 monomer 1 * 30200, SDS-PAGE Caloglossa continua

Synonyms

EC Number Synonyms Comment Organism
3.1.3.22 M1Pase
-
 Caloglossa continua

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.22 7.4
-
-
 Caloglossa continua

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.22 6.5 8.5 pH 6.5: about 60% of maximal activity, pH 8.5: about 60% of maximal activity Caloglossa continua

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.22 Caloglossa continua isoelectric focusing
-
 4.8