BRENDA - Enzyme Database show

In vitro effects of fat, FA, and cholesterol on sphingomyelin hydrolysis induced by rat intestinal alkaline sphingomyelinase

Liu, J.J.; Nilsson, A.; Duan, R.D.; Lipids 37, 469-474 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.12
1-oleoyl-rac-glycerol
approx. 20% stimulation at 0.4-0.8 mM, inhibition above
Rattus norvegicus
3.1.4.12
1-stearoyl-rac-glycerol
approx. 10% stimulation at 0.8 mM, inhibition above
Rattus norvegicus
3.1.4.12
arachidonic acid
approx. 20% stimulation at 0.4-0.8 mM, inhibition above
Rattus norvegicus
3.1.4.12
linoleic acid
approx. 20% stimulation at 0.8 mM, inhibition above
Rattus norvegicus
3.1.4.12
linolenic acid
approx. 30% stimulation at 0.8-1.6 mM, inhibition above
Rattus norvegicus
3.1.4.12
oleic acid
approx. 40% stimulation at 0.8 mM, inhibition above
Rattus norvegicus
3.1.4.12
stearic acid
approx. 20% stimulation at 0.4-0.8 mM, inhibition above
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
1,2-dioleoyl-sn-glycerol
approx. 40% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
1,2-distearoyl-rac-glycerol
approx. 30% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
1-oleoyl-rac-glycerol
slight stimulation below 0.8 mM, approx. 50% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
1-stearoyl-rac-glycerol
slight stimulation below 0.8 mM, approx. 10% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
arachidonic acid
slight stimulation below 0.8 mM, approx. 50% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
ceramide
strong inhibition in the presence of 0.4 mM linoleic acid
Rattus norvegicus
3.1.4.12
cholesterol
strong inhibition in the presence of 0.4 mM linoleic acid
Rattus norvegicus
3.1.4.12
linoleic acid
slight stimulation below 0.8 mM, approx. 45% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
linolenic acid
stimulation below 0.8 mM, approx. 15% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
oleic acid
stimulation below 0.8 mM, approx. 10% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
stearic acid
slight stimulation below 0.8 mM, approx. 10% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
triolein
approx. 30% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
tristearin
approx. 35% inhibition at 3.2 mM
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.12
sphingomyelin + H2O
Rattus norvegicus
-
N-acylsphingosine + choline phosphate
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.12
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.12
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.12
intestine
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.12
sphingomyelin + H2O
-
653103
Rattus norvegicus
N-acylsphingosine + choline phosphate
-
653103
Rattus norvegicus
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.12
1-oleoyl-rac-glycerol
approx. 20% stimulation at 0.4-0.8 mM, inhibition above
Rattus norvegicus
3.1.4.12
1-stearoyl-rac-glycerol
approx. 10% stimulation at 0.8 mM, inhibition above
Rattus norvegicus
3.1.4.12
arachidonic acid
approx. 20% stimulation at 0.4-0.8 mM, inhibition above
Rattus norvegicus
3.1.4.12
linoleic acid
approx. 20% stimulation at 0.8 mM, inhibition above
Rattus norvegicus
3.1.4.12
linolenic acid
approx. 30% stimulation at 0.8-1.6 mM, inhibition above
Rattus norvegicus
3.1.4.12
oleic acid
approx. 40% stimulation at 0.8 mM, inhibition above
Rattus norvegicus
3.1.4.12
stearic acid
approx. 20% stimulation at 0.4-0.8 mM, inhibition above
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
1,2-dioleoyl-sn-glycerol
approx. 40% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
1,2-distearoyl-rac-glycerol
approx. 30% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
1-oleoyl-rac-glycerol
slight stimulation below 0.8 mM, approx. 50% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
1-stearoyl-rac-glycerol
slight stimulation below 0.8 mM, approx. 10% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
arachidonic acid
slight stimulation below 0.8 mM, approx. 50% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
ceramide
strong inhibition in the presence of 0.4 mM linoleic acid
Rattus norvegicus
3.1.4.12
cholesterol
strong inhibition in the presence of 0.4 mM linoleic acid
Rattus norvegicus
3.1.4.12
linoleic acid
slight stimulation below 0.8 mM, approx. 45% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
linolenic acid
stimulation below 0.8 mM, approx. 15% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
oleic acid
stimulation below 0.8 mM, approx. 10% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
stearic acid
slight stimulation below 0.8 mM, approx. 10% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
triolein
approx. 30% inhibition at 3.2 mM
Rattus norvegicus
3.1.4.12
tristearin
approx. 35% inhibition at 3.2 mM
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.12
sphingomyelin + H2O
Rattus norvegicus
-
N-acylsphingosine + choline phosphate
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.12
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.12
intestine
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.12
sphingomyelin + H2O
-
653103
Rattus norvegicus
N-acylsphingosine + choline phosphate
-
653103
Rattus norvegicus
?