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Literature summary extracted from

  • Dubouix, A.; Nieto, M.; Fauvel, J.; Chap, H.; Marty, N.; Salles, J.P.; Gaits, F.
    A simple and reliable method for rapid production and purification of Pseudomonas aeruginosa haemolytic phospholipase C (2004), Lett. Appl. Microbiol., 38, 191-196.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 Zn2+ required, 10 mM Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.3 production of the recombinant protein in Escherichia coli is no more successful because of frequent inclusion bodies and accumulation of inactive phospholipase C in the periplasmic space Pseudomonas aeruginosa

General Stability

EC Number General Stability Organism
3.1.4.3 CHAPS, 20 mM, or Triton X-100, 20 mM, no loss of activity Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 additional information the recombinant enzyme is resistant to 20 mM CHAPS or 20 mM Triton X-100 Pseudomonas aeruginosa
3.1.4.3 Zn2+ recombinant enzyme Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 72800
-
 x * 72800, truncated recombinant protein with His6-tag, SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Pseudomonas aeruginosa
-
-
-
3.1.4.3 Pseudomonas aeruginosa
-
 recombinant enzyme, production in Schizosaccharomyces pombe
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 method for production of recombinant enzyme in Schizosaccharomyces pombe Pseudomonas aeruginosa
3.1.4.3 recombinant Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 10
-
-
 Pseudomonas aeruginosa
3.1.4.3 1940
-
 pH 7.3, 37°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 p-nitrophenylphosphorylcholine + H2O
-
 Pseudomonas aeruginosa p-nitrophenol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O substrate from egg yolk Pseudomonas aeruginosa 1,2-diacylglycerol + phosphorylcholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.3 ? x * 72800, truncated recombinant protein with His6-tag, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.1.4.3 haemolytic phospholipase C
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.3 95
-
 1 min, complete loss of activity Pseudomonas aeruginosa