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Literature summary extracted from

  • Ozment, C.; Barchue, J.; DeLucas, L.J.; Chattopadhyay, D.
    Structural study of Escherichia coli NAD synthetase: overexpression, purification, crystallization, and preliminary crystallographic analysis (1999), J. Struct. Biol., 127, 279-282.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.5 overexpression in strain BL21(DE3) as HIs-tagged enzyme Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.1.5 28 mg/ml purified recombinant enzyme, pure or in complex with substrates NAD+ and ATP, hanging-drop vapour diffusion method, 22°C room temperature, protein in 0.01 M HEPES, pH 7.5, 5 mM MgCl2, 3 mM DTT, precipitant solution: 0.1 M HEPES, pH 7.5, 1.5 M lithium sulfate, X-ray diffraction structure determination at 2.0 A resolution, compex structure determination via molecular replacement method Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.5 Mg2+ slightly inhibiting above 5 mM Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.5 Mg2+ required at 1.5 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.5 35000
-
 x * 35000, recombinant enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + glutamine Escherichia coli
-
 AMP + diphosphate + glutamate + NAD+
-
 ?
6.3.1.5 ATP + deamido-NAD+ + NH3 Escherichia coli
-
 AMP + diphosphate + NAD+
-
 ?
6.3.1.5 additional information Escherichia coli 2 possible substrates, NH3 or glutamine, to perform the final step in the Preiss-Handler pathway for NAD+ biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.5 recombinantly overexpressed enzyme, to homogeneity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + glutamine
-
 Escherichia coli AMP + diphosphate + glutamate + NAD+
-
 ?
6.3.1.5 ATP + deamido-NAD+ + NH3
-
 Escherichia coli AMP + diphosphate + NAD+
-
 ?
6.3.1.5 additional information 2 possible substrates, NH3 or glutamine, to perform the final step in the Preiss-Handler pathway for NAD+ biosynthesis Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.5 ? x * 35000, recombinant enzyme, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.3.1.5 NAD synthase
-
 Escherichia coli
6.3.1.5 NAD synthetase
-
 Escherichia coli
6.3.1.5 Nicotinamide adenine dinucleotide synthetase
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.5 8 8.5
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.1.5 additional information
-
 active in a broad pH range Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.5 ATP
-
 Escherichia coli