BRENDA - Enzyme Database

Structural studies on a 2,3-diphosphoglycerate independent phosphoglycerate mutase from Bacillus stearothermophilus

Chander, M.; Setlow, P.; Lamani, E.; Jedrzejas, M.J.; J. Struct. Biol. 126, 156-165 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.12
expression of native and selenomethionyl phosphoglycerate mutase in Escherichia coli
Geobacillus stearothermophilus
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.4.2.12
methionyl and selenomethionyl phosphoglycerate mutase, vapor drop hanging diffusion, mixing equal volumes of protein solution, i.e. 50 mg/ml protein in 150 mM 3-phosphoglycerate, pH 7.4 and reservoir solution containing 2 M ammonium sulfate, 25 mM zinc acetate, 20 mM cesium chloride, 15 mM 2-mercaptoethanol, 3% polyethylene glycol 200 and 50 mM Tris-HCl, pH 7.4, crystals diffract to 2.5 A resolution
Geobacillus stearothermophilus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
4.4
-
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide
pH 7.4, 1 mM Mn2+
Geobacillus stearothermophilus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Mn2+
required for activity
Geobacillus stearothermophilus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
60000
-
x * 60000, SDS-PAGE
Geobacillus stearothermophilus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Geobacillus stearothermophilus
-
3-phospho-D-glycerate
-
Geobacillus stearothermophilus
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Geobacillus stearothermophilus
Q9X519
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.12
recombinant phosphoglycerate mutase
Geobacillus stearothermophilus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.2.12
500
-
selenomethionyl-phosphoglycerate mutase
Geobacillus stearothermophilus
5.4.2.12
1500
-
methionyl-phosphoglycerate mutase
Geobacillus stearothermophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
653052
Geobacillus stearothermophilus
3-phospho-D-glycerate
-
653052
Geobacillus stearothermophilus
r
Subunits
EC Number
Subunits
Commentary
Organism
5.4.2.12
?
x * 60000, SDS-PAGE
Geobacillus stearothermophilus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
8
-
280fold and 25fold increase in activity if pH is raised from 6.0 to 8.0 in the presence of 0.004 mM Mn2+ and 1 mM Mn2+, respectively
Geobacillus stearothermophilus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate phosphoglycerate mutase
Geobacillus stearothermophilus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
5.4.2.12
Geobacillus stearothermophilus
isoelectric focusing
-
6
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
expression of native and selenomethionyl phosphoglycerate mutase in Escherichia coli
Geobacillus stearothermophilus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate phosphoglycerate mutase
Geobacillus stearothermophilus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.4.2.12
methionyl and selenomethionyl phosphoglycerate mutase, vapor drop hanging diffusion, mixing equal volumes of protein solution, i.e. 50 mg/ml protein in 150 mM 3-phosphoglycerate, pH 7.4 and reservoir solution containing 2 M ammonium sulfate, 25 mM zinc acetate, 20 mM cesium chloride, 15 mM 2-mercaptoethanol, 3% polyethylene glycol 200 and 50 mM Tris-HCl, pH 7.4, crystals diffract to 2.5 A resolution
Geobacillus stearothermophilus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
4.4
-
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide
pH 7.4, 1 mM Mn2+
Geobacillus stearothermophilus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Mn2+
required for activity
Geobacillus stearothermophilus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
60000
-
x * 60000, SDS-PAGE
Geobacillus stearothermophilus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Geobacillus stearothermophilus
-
3-phospho-D-glycerate
-
Geobacillus stearothermophilus
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
recombinant phosphoglycerate mutase
Geobacillus stearothermophilus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.2.12
500
-
selenomethionyl-phosphoglycerate mutase
Geobacillus stearothermophilus
5.4.2.12
1500
-
methionyl-phosphoglycerate mutase
Geobacillus stearothermophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
653052
Geobacillus stearothermophilus
3-phospho-D-glycerate
-
653052
Geobacillus stearothermophilus
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.2.12
?
x * 60000, SDS-PAGE
Geobacillus stearothermophilus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
8
-
280fold and 25fold increase in activity if pH is raised from 6.0 to 8.0 in the presence of 0.004 mM Mn2+ and 1 mM Mn2+, respectively
Geobacillus stearothermophilus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
5.4.2.12
Geobacillus stearothermophilus
isoelectric focusing
-
6