Literature summary extracted from
Valerio, A.A.; Corradini, A.C.; Panunto, P.C.; Mello, S.M.; Hyslop, S.
Purification and characterization of a phosphodiesterase from Bothrops alternatus snake venom (2002), J. Protein Chem., 21, 495-503.
General Stability
EC Number |
General Stability |
Organism |
---|
3.1.15.1 |
repeated freeze-thaw cycles lead to loss of enzyme activity |
Bothrops alternatus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.15.1 |
2-mercaptoethanol |
10 mM, 20% of inhibition |
Bothrops alternatus |
|
3.1.15.1 |
CuSO4 |
10 mM, 90% of inhibition |
Bothrops alternatus |
|
3.1.15.1 |
EDTA |
10 mM, 90% of inhibition |
Bothrops alternatus |
|
3.1.15.1 |
MgSO4 |
100 mM, 40% of inhibition |
Bothrops alternatus |
|
3.1.15.1 |
MnCl2 |
10 mM, 50% of inhibition |
Bothrops alternatus |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.15.1 |
2.69 |
- |
bis(p-nitrophenyl)-phosphate |
37°C, pH 8.9 |
Bothrops alternatus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.15.1 |
Co2+ |
10 mM, 170% of activity |
Bothrops alternatus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.15.1 |
105000 |
- |
PAGE |
Bothrops alternatus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.15.1 |
Bothrops alternatus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.15.1 |
- |
Bothrops alternatus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
3.1.15.1 |
25°C, 0.1 M NaOH-glycine, pH 8.9, 72 h, stable |
Bothrops alternatus |
3.1.15.1 |
37°C, 0.1 M NaOH-glycine, pH 8.9, 72 h, stable |
Bothrops alternatus |
3.1.15.1 |
4°C, 0.1 M NaOH-glycine, pH 8.9, 72 h, stable |
Bothrops alternatus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.15.1 |
bis(p-nitrophenyl)-phosphate + H2O |
- |
Bothrops alternatus |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.15.1 |
monomer |
1 * 105000, SDS-PAGE |
Bothrops alternatus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.15.1 |
60 |
- |
- |
Bothrops alternatus |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.1.15.1 |
70 |
- |
1 min, complete loss of activity |
Bothrops alternatus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.15.1 |
7.5 |
9.5 |
- |
Bothrops alternatus |