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Literature summary extracted from

  • Valerio, A.A.; Corradini, A.C.; Panunto, P.C.; Mello, S.M.; Hyslop, S.
    Purification and characterization of a phosphodiesterase from Bothrops alternatus snake venom (2002), J. Protein Chem., 21, 495-503.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.15.1 repeated freeze-thaw cycles lead to loss of enzyme activity Bothrops alternatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.15.1 2-mercaptoethanol 10 mM, 20% of inhibition Bothrops alternatus
3.1.15.1 CuSO4 10 mM, 90% of inhibition Bothrops alternatus
3.1.15.1 EDTA 10 mM, 90% of inhibition Bothrops alternatus
3.1.15.1 MgSO4 100 mM, 40% of inhibition Bothrops alternatus
3.1.15.1 MnCl2 10 mM, 50% of inhibition Bothrops alternatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.15.1 2.69
-
bis(p-nitrophenyl)-phosphate 37°C, pH 8.9 Bothrops alternatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.15.1 Co2+ 10 mM, 170% of activity Bothrops alternatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.15.1 105000
-
PAGE Bothrops alternatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.15.1 Bothrops alternatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.15.1
-
Bothrops alternatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
3.1.15.1 25°C, 0.1 M NaOH-glycine, pH 8.9, 72 h, stable Bothrops alternatus
3.1.15.1 37°C, 0.1 M NaOH-glycine, pH 8.9, 72 h, stable Bothrops alternatus
3.1.15.1 4°C, 0.1 M NaOH-glycine, pH 8.9, 72 h, stable Bothrops alternatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.15.1 bis(p-nitrophenyl)-phosphate + H2O
-
Bothrops alternatus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.15.1 monomer 1 * 105000, SDS-PAGE Bothrops alternatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.15.1 60
-
-
Bothrops alternatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.15.1 70
-
1 min, complete loss of activity Bothrops alternatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.15.1 7.5 9.5
-
Bothrops alternatus