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Literature summary extracted from

  • Bonfim, V.L.; Toyama, M.H.; Novello, J.C.; Hyslop, S.; Oliveira, C.R.; Rodrigues-Simioni, L.; Marangoni, S.
    Isolation and enzymatic characterization of a basic phospholipase A2 from Bothrops jararacussu snake venom (2001), J. Protein Chem., 20, 239-245.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 crotapotin
-
 Bothrops jararacussu
3.1.1.4 Cu2+ 10 mM Bothrops jararacussu
3.1.1.4 Zn2+ 10 mM Bothrops jararacussu

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 1.7
-
 4-nitro-3-(octanoyloxy)benzoic acid pH 8, 37°C Bothrops jararacussu

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+ requirement, optimal concentration: 10 mM Bothrops jararacussu
3.1.1.4 Mg2+ 10 mM Mg2+ increases the activity slightly in the presence of 1 mM Ca2+ Bothrops jararacussu
3.1.1.4 Mn2+ 10 mM Mn2+ increases the activity slightly in the presence of 1 mM Ca2+ Bothrops jararacussu

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 15000
-
 SDS-PAGE Bothrops jararacussu
3.1.1.4 30000
-
 SDS-PAGE Bothrops jararacussu

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Bothrops jararacussu
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4
-
 Bothrops jararacussu

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 venom
-
 Bothrops jararacussu
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 4-nitro-3-(octanoyloxy)benzoic acid + H2O synthetic substrate Bothrops jararacussu 3-hydroxy-4-nitrobenzoate + octanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 multimer enzyme forms multimeric complexes of 30000 Da, 45000 Da and 60000 Da Bothrops jararacussu

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 BthTX
-
 Bothrops jararacussu

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.4 35 45
-
 Bothrops jararacussu

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 8.2
-
-
 Bothrops jararacussu