Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hountondji, C.; Beauvallet, C.; Pernollet, J.C.; Blanquet, S.
    Enzyme-induced covalent modification of methionyl-tRNA synthetase from Bacillus stearothermophilus by methionyl-adenylate: identification of the labeled amino acid residues by matrix-assisted laser desorption-ionization mass spectrometry (2000), J. Protein Chem., 19, 563-568.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.10 additional information construction of a truncated enzyme form with 25% reduced activity compared to the wild-type enzyme Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.10 Mg2+
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.10 62000
-
 recombinant truncated mutant, mass spectrometry Geobacillus stearothermophilus
6.1.1.10 149000
-
 recombinant wild-type enzyme, mass spectrometry Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Geobacillus stearothermophilus
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Geobacillus stearothermophilus
-
 MS534, purified recombinant dimeric wild-type and monomeric truncated mutant enzymes expressed in Escherichia coli
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.10 ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet reaction mechanism, Lys261, Lys295, and Lys301 are located in the catalytic crevice of the enzyme Geobacillus stearothermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Geobacillus stearothermophilus AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet two-step reaction mechanism, enzyme forms the reaction intermediate L-methionyl-adenylate, which covalently methionylates the enzyme at the epsilon-amino group of a lysine residue, inducing structural modifcation, 4.3 and 2.2 mol of Met are incorporated by 1 mol of wild-type enzyme and truncated mutant enzyme, respectively Geobacillus stearothermophilus AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 additional information enzyme also performs the ATP-diphosphate exchange reaction Geobacillus stearothermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.10 dimer recombinant wild-type, mass spectrometry Geobacillus stearothermophilus
6.1.1.10 monomer 1 * 62000, recombinant truncated mutant enzyme, mass spectrometry Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 Methionine translase
-
 Geobacillus stearothermophilus
6.1.1.10 Methionine--tRNA ligase
-
 Geobacillus stearothermophilus
6.1.1.10 Methionyl tRNA synthetase
-
 Geobacillus stearothermophilus
6.1.1.10 Methionyl-transfer ribonucleate synthetase
-
 Geobacillus stearothermophilus
6.1.1.10 Methionyl-transfer ribonucleic acid synthetase
-
 Geobacillus stearothermophilus
6.1.1.10 Methionyl-transfer RNA synthetase
-
 Geobacillus stearothermophilus
6.1.1.10 MetRS
-
 Geobacillus stearothermophilus
6.1.1.10 Synthetase, methionyl-transfer ribonucleate
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.10 37
-
 assay at Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.10 additional information
-
 thermostable enzyme Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.10 8
-
 assay at Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
 Geobacillus stearothermophilus