EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.5 | 2-mercaptoethanol | 1.4fold at 50 mM, 1.6fold activation at 100 mM | Pseudomonas fluorescens | |
3.5.5.5 | phenylacetonitrile | absolutely required as growth nitrogen source substrate for induction of the enzyme, cannot be substituted by ammonia or epsilon-caprolactam | Pseudomonas fluorescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.5 | Ag+ | complete inhibition at 1 mM | Pseudomonas fluorescens | |
3.5.5.5 | Cu2+ | complete inhibition at 1 mM | Pseudomonas fluorescens | |
3.5.5.5 | Fe2+ | complete inhibition at 10 mM | Pseudomonas fluorescens | |
3.5.5.5 | Fe3+ | complete inhibition at 1 mM | Pseudomonas fluorescens | |
3.5.5.5 | additional information | no inhibition by KCN, EDTA, D-cycloserine, and Mn2+ | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.5.5 | 0.065 | - |
phenylacetonitrile | pH 7.4, 30°C | Pseudomonas fluorescens | |
3.5.5.5 | 0.087 | - |
rac 2-(methoxy)-mandelonitrile | pH 7.4, 30°C | Pseudomonas fluorescens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.5 | additional information | enzyme does not seem to require metals for activity | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 13000 | - |
gel filtration | Pseudomonas fluorescens |
3.5.5.5 | 38000 | - |
1 * 57000 + 1 * 40000 + 1 * 38000, SDS-PAGE | Pseudomonas fluorescens |
3.5.5.5 | 40000 | - |
1 * 57000 + 1 * 40000 + 1 * 38000, SDS-PAGE | Pseudomonas fluorescens |
3.5.5.5 | 57000 | - |
1 * 57000 + 1 * 40000 + 1 * 38000, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.5 | Pseudomonas fluorescens | - |
DSM 7155, inducible enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.5.5 | partial, 259fold | Pseudomonas fluorescens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 90 | - |
partially purified enzyme | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.5 | 2-phenylpropionitrile + H2O | low activity, 2% activity compared to phenylacetonitrile | Pseudomonas fluorescens | 2-phenylpropionate + NH3 | - |
? | |
3.5.5.5 | additional information | no activity with benzonitrile, 2-cyanotoluene, 3-cyanotoluene, 3-cyanobenzothiophene, propionitrile, butyronitrile, and valeronitrile, 2-phenylbutyronitrile is a poor substrate, enzyme is enantioselective and specific for arylacetonitriles | Pseudomonas fluorescens | ? | - |
? | |
3.5.5.5 | phenylacetonitrile + 2 H2O | best substrate | Pseudomonas fluorescens | phenylacetate + NH3 | - |
? | |
3.5.5.5 | rac 2-(methoxy)-mandelonitrile + H2O | - |
Pseudomonas fluorescens | (R)-2-(methoxy)-mandelic acid + (S)-2-(methoxy)-mandelic acid + NH3 | 10% activity compared to phenylacetonitrile, 92% of the product is the (R)-enantiomer when the overall conversion rate is 50%, at 85% overall conversion, the level of formed (R)-enantiomer is 27% | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.5.5 | trimer | 1 * 57000 + 1 * 40000 + 1 * 38000, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.5.5 | nitrilase | - |
Pseudomonas fluorescens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 55 | - |
- |
Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 9 | - |
- |
Pseudomonas fluorescens |