EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.3.7 | overexpression of wild-type and mutant enzymes in strain XL1-Blue | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.3.7 | purified recombinant wild-type and mutant enzymes, hanging or sitting drop vapour diffusion method, at 4°C and 21°C, 0.006 ml protein solutions: about 10 mg/ml protein, 1.8 M K2PO4, pH 10.0, N-octyl-beta-R-glucopyranoside 6% w/v, + 2 ml reservoir solution: 1.8 M K2PO4, pH 10.0, 3-4 days, X-ray diffraction structure determination and analysis at 2.35-2.5 A resolution | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.3.7 | additional information | detailed structural properties of the mutant enzymes based on the crystal structure models | Escherichia coli |
4.3.3.7 | T44V | site-directed mutagenesis, reduced activity, structure is similar to the wild-type enzyme | Escherichia coli |
4.3.3.7 | Y107F | site-directed mutagenesis, reduced activity, structure is similar to the wild-type enzyme | Escherichia coli |
4.3.3.7 | Y133F | site-directed mutagenesis, reduced activity, structure is similar to the wild-type enzyme | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.7 | 0.08 | - |
pyruvate | mutant T44V, pH 8.0, 30°C | Escherichia coli | |
4.3.3.7 | 0.09 | - |
(S)-aspartate 4-semialdehyde | mutant T44V, pH 8.0, 30°C | Escherichia coli | |
4.3.3.7 | 0.11 | - |
(S)-aspartate 4-semialdehyde | wild-type enzyme, pH 8.0, 30°C | Escherichia coli | |
4.3.3.7 | 0.16 | - |
pyruvate | mutant Y107F, pH 8.0, 30°C | Escherichia coli | |
4.3.3.7 | 0.26 | - |
pyruvate | wild-type enzyme, pH 8.0, 30°C | Escherichia coli | |
4.3.3.7 | 0.58 | - |
(S)-aspartate 4-semialdehyde | mutant Y107F, pH 8.0, 30°C | Escherichia coli | |
4.3.3.7 | 2.7 | - |
(S)-aspartate 4-semialdehyde | mutant Y133F, pH 8.0, 30°C | Escherichia coli | |
4.3.3.7 | 35 | - |
pyruvate | mutant Y133F, pH 8.0, 30°C | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.7 | (S)-aspartate 4-semialdehyde + pyruvate | Escherichia coli | branch point reaction in the biosynthesis of lysine | dihydrodipicolinate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.3.7 | Escherichia coli | P0A6L2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.3.7 | recombinant wild-type 5.8fold, recombinant mutant Y107F 17.6fold, recombinant mutant T44V 15.8fold, and recombinant mutant Y133F 18fold | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.3.3.7 | pyruvate + L-aspartate-4-semialdehyde = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | ping-pong kinetic and catalytic mechanism involves a catalytic triad which consists of Tyr133, Thr44, and Tyr107, overview | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.3.3.7 | 0.0034 | - |
purified mutant T44V | Escherichia coli |
4.3.3.7 | 0.008 | - |
purified mutant Y133F | Escherichia coli |
4.3.3.7 | 0.27 | - |
purified mutant Y107F | Escherichia coli |
4.3.3.7 | 1.81 | - |
purified wild-type enzyme | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.7 | (S)-aspartate 4-semialdehyde + pyruvate | - |
Escherichia coli | dihydrodipicolinate + H2O | - |
? | |
4.3.3.7 | (S)-aspartate 4-semialdehyde + pyruvate | branch point reaction in the biosynthesis of lysine | Escherichia coli | dihydrodipicolinate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.3.3.7 | tetramer | homotetramer of (alpha/beta)8 barrels, each containing one active site, crystal structure | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.3.7 | DHDPS | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.7 | 0.12 | - |
(S)-aspartate 4-semialdehyde | mutant T44V, pH 8.0, 30°C | Escherichia coli | |
4.3.3.7 | 0.7 | - |
(S)-aspartate 4-semialdehyde | mutant Y133F, pH 8.0, 30°C | Escherichia coli | |
4.3.3.7 | 10.8 | - |
(S)-aspartate 4-semialdehyde | mutant Y107F, pH 8.0, 30°C | Escherichia coli | |
4.3.3.7 | 124 | - |
(S)-aspartate 4-semialdehyde | wild-type enzyme, pH 8.0, 30°C | Escherichia coli |