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Literature summary extracted from

  • Smith, S.A.; Tabita, F.R.
    Positive and negative selection of mutant forms of prokaryotic (cyanobacterial) ribulose-1,5-bisphosphate carboxylase/oxygenase (2003), J. Mol. Biol., 331, 557-569.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.39 rbcLS genes, Rhodobacter capsulatus as host Synechococcus sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.39
-
 Synechococcus sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.39 D103E site-directed mutagenesis, mutant enzyme is capable of supporting only slight growth of strain SBI-II- on photoautotrophic plates Synechococcus sp.
4.1.1.39 D103N site-directed mutagenesis, mutant enzyme is capable of supporting only slight growth of strain SBI-II- on photoautotrophic plates Synechococcus sp.
4.1.1.39 D103V site-directed mutagenesis, mutant enzyme is capable of supporting only slight growth of strain SBI-II- on photoautotrophic plates Synechococcus sp.
4.1.1.39 F342V site-directed mutagenesis, mutant enzyme is shown to support the growth of strain SBI-II- on plates under aerobic chemoautotrophic growth conditions while the wild-type enzyme does not Synechococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.39 55000
-
 8 * 55000 + 8 * 12000-15000 Synechococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O Synechococcus sp. Calvin-Benson-Bassham reductive pentose phosphate pathway, first step in net photosynthetic CO2 assimilation and photorespiratory carbon oxidation, most abundant protein on earth 3-phospho-D-glycerate
-
 ?
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O Synechococcus sp. PCC6301 Calvin-Benson-Bassham reductive pentose phosphate pathway, first step in net photosynthetic CO2 assimilation and photorespiratory carbon oxidation, most abundant protein on earth 3-phospho-D-glycerate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Synechococcus sp.
-
 PCC6301
-
4.1.1.39 Synechococcus sp. PCC6301
-
 PCC6301
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Synechococcus sp. 3-phospho-D-glycerate
-
 ?
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O Calvin-Benson-Bassham reductive pentose phosphate pathway, first step in net photosynthetic CO2 assimilation and photorespiratory carbon oxidation, most abundant protein on earth Synechococcus sp. 3-phospho-D-glycerate
-
 ?
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Synechococcus sp. PCC6301 3-phospho-D-glycerate
-
 ?
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O Calvin-Benson-Bassham reductive pentose phosphate pathway, first step in net photosynthetic CO2 assimilation and photorespiratory carbon oxidation, most abundant protein on earth Synechococcus sp. PCC6301 3-phospho-D-glycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.39 hexadecamer 8 * 55000 + 8 * 12000-15000 Synechococcus sp.

Synonyms

EC Number Synonyms Comment Organism
4.1.1.39 ribulose-1,5-bisphosphate carboxylase/oxygenase
-
 Synechococcus sp.
4.1.1.39 Rubisco
-
 Synechococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.39 1.3
-
 D-ribulose-1,5-bisphosphate pH 8.0, 23°C, mutant D103E Synechococcus sp.
4.1.1.39 1.8
-
 D-ribulose-1,5-bisphosphate pH 8.0, 23°C, mutant D103N Synechococcus sp.
4.1.1.39 2.4
-
 D-ribulose-1,5-bisphosphate pH 8.0, 23°C, mutant F342V and D103V Synechococcus sp.
4.1.1.39 3.5
-
 D-ribulose-1,5-bisphosphate pH 8.0, 23°C, wild-type Synechococcus sp.