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Literature summary extracted from

  • Torres-Larios, A.; Sankaranarayanan, R.; Rees, B.; Dock-Bregeon, A.C.; Moras, D.
    Conformational movements and cooperativity upon amino acid, ATP and tRNA binding in threonyl-tRNA synthetase (2003), J. Mol. Biol., 331, 201-211.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.3 overexpression in Escherichia coli Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.3 enzyme complexed with ATP and threonine and complexed with substrate analogue 5'-O-(N-(L-threonyl)-sulfamoyl)adenosine, hanging drop method, 15 mg/ml protein in 20 mM HEPES, pH 7.2, 2 mM MgCl2, 100 mM KCl, in a 1:1:1 mixture with ligand solution containing 10 mM MgCl2, 10 mM ATP or 1 mM Thr-AMS, and well solution containing 50 mM Tris-HCl, pH 7.5-8.5, PEG 8000 12-14% w/v, and ammonium acetate or potassium chloride in the range 0.2-0.4 M, 2-3 days at 4°C, X-ray diffraction structure determination at resolution 2.8-3.2 A, structure analysis, modeling Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.3 Mg2+ required Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr Staphylococcus aureus
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.3 Staphylococcus aureus Q8NW68
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.3 recombinant from Escherichia coli Staphylococcus aureus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.3 ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr substrate binding and mechanism Staphylococcus aureus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr
-
 Staphylococcus aureus AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr substrate binding induces conformational changes Staphylococcus aureus AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.3 Synthetase, threonyl-transfer ribonucleate
-
 Staphylococcus aureus
6.1.1.3 Threonine translase
-
 Staphylococcus aureus
6.1.1.3 Threonine--tRNA ligase
-
 Staphylococcus aureus
6.1.1.3 Threonine-transfer ribonucleate synthetase
-
 Staphylococcus aureus
6.1.1.3 Threonyl-ribonucleic synthetase
-
 Staphylococcus aureus
6.1.1.3 Threonyl-transfer ribonucleate synthetase
-
 Staphylococcus aureus
6.1.1.3 Threonyl-transfer ribonucleic acid synthetase
-
 Staphylococcus aureus
6.1.1.3 Threonyl-transfer RNA synthetase
-
 Staphylococcus aureus
6.1.1.3 Threonyl-tRNA synthetase
-
 Staphylococcus aureus
6.1.1.3 ThrRS
-
 Staphylococcus aureus
6.1.1.3 TRS
-
 Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.3 7.7
-
 assay at Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.3 ATP
-
 Staphylococcus aureus