Literature summary extracted from

Kim, J.S.; Groll, M.; Musiol, H.J.; Behrendt, R.; Kaiser, M.; Moroder, L.; Huber, R.; Brandstetter, H.
Navigation inside a protease: substrate selection and product exit in the tricorn protease from Thermoplasma acidophilum (2002), J. Mol. Biol., 324, 1041-1050.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.4.21.B34	enzyme in complex with inhibitor: decanoyl-Arg-Val-Arg-Lys-chloromethyl ketone, Asp-Gln-Thr-Gln-Lys-Gln-Tyr-Gln-Glu-Leu-Thr-Phe-Phe-chloromethyl ketone or benzyloxycarbonyl-Phe-PSI[CO-CONH]Arg-Glu-Phe-OH, hanging drop vapour diffusion method	Thermoplasma acidophilum

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.4.21.B34	L184C	increase in activity with benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin and insulin B chain to more than 150% of the wild-type activity, no activity with casein	Thermoplasma acidophilum
3.4.21.B34	R131E/R132E	slight decrease in activity with benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin, strong decrease in activity with insulin B chain, barely detectable activity with casein	Thermoplasma acidophilum
3.4.21.B34	R414C/A643C	decrease in activity with benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin to 70% of wild-type activity, decrease in activity with insulin B chain as substrate to 50% of wild-type activity, no activity with casein	Thermoplasma acidophilum

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.21.B34	Asp-Gln-Thr-Gln-Lys-Gln-Tyr-Gln-Glu-Leu-Thr-Phe-Phe-chloromethyl ketone	-	Thermoplasma acidophilum
3.4.21.B34	benzyloxycarbonyl-Phe-PSI[CO-CONH]Arg-Glu-Phe-OH	-	Thermoplasma acidophilum
3.4.21.B34	decanoyl-Arg-Val-Arg-Lys-chloromethyl ketone	-	Thermoplasma acidophilum

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.21.B34	Thermoplasma acidophilum	P96086	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.21.B34	benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O	-	Thermoplasma acidophilum	benzoyl-Val-Gly-Arg + 7-amino-4-methylcoumarin	-	?
3.4.21.B34	casein + H2O	-	Thermoplasma acidophilum	?	-	?
3.4.21.B34	insulin B chain + H2O	-	Thermoplasma acidophilum	?	-	?

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Release 2023.1 (January 2023)