BRENDA - Enzyme Database

Crystal structure of the Escherichia coli RNA degradosome component enolase

Kuhnel, K.; Luisi, B.F.; J. Mol. Biol. 313, 583-592 (2001)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.11
using the sitting drop method, co-crystallized with Mg2+
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
as part of the degradosome complex
Escherichia coli
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
45545
-
2 * 45545, mass spectrometry and X-ray crystallography
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
using gel filtration chromatography
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
260
-
pH 8.1, room temperature
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
652871
Escherichia coli
phosphoenolpyruvate
-
652871
Escherichia coli
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 45545, mass spectrometry and X-ray crystallography
Escherichia coli
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.11
using the sitting drop method, co-crystallized with Mg2+
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
as part of the degradosome complex
Escherichia coli
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
45545
-
2 * 45545, mass spectrometry and X-ray crystallography
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
using gel filtration chromatography
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
260
-
pH 8.1, room temperature
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
652871
Escherichia coli
phosphoenolpyruvate
-
652871
Escherichia coli
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 45545, mass spectrometry and X-ray crystallography
Escherichia coli