Literature summary extracted from
Yoon, H.J.; Hashimoto, W.; Miyake, O.; Murata, K.; Mikami, B.
Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution (2001), J. Mol. Biol., 307, 9-16.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.2.2.3 |
overexpression of isozyme A1-III in Bacillus subtilis |
Sphingomonas sp. |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.2.2.3 |
8 mg/ml purified isozyme A1-III complexed with trisaccharide product 4-deoxy-L-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid, hanging drop vapour diffusion method, 0.1 M HEPES, pH 7.5, containg 48% PEG w/v saturated ammonium sulfate, 290 mM trisaccharide, 20°C, X-ray diffraction structure determination and analysis at 2.0 A resolution |
Sphingomonas sp. |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.2.3 |
Sphingomonas sp. |
- |
isozyme A1-III |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.2.3 |
recombinant isozyme A1-III from Bacillus subtilis |
Sphingomonas sp. |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
4.2.2.3 |
R2-beta-D-mannuronic acid-R1 = R2-OH + 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-R1 |
substrate binding site structure, active site cleft, catalytic mechanism |
Sphingomonas sp. |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.2.3 |
poly-beta-1,4-D-mannuronate |
- |
Sphingomonas sp. |
4-deoxy-L-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.2.2.3 |
alginate lyase |
- |
Sphingomonas sp. |