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Literature summary extracted from

  • Schelp, E.; Worley, S.; Monzingo, A.F.; Ernst, S.; Robertus, J.D.
    pH-induced structural changes regulate histidine decarboxylase activity in Lactobacillus 30a (2001), J. Mol. Biol., 306, 727-732.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.22 pyruvate
-
 Lactobacillus sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.22 crystal structure of active HDC at pH 4.8 at 2.5 A resolution, crystal structure of less active HDC at pH 8.0 at 2.7 A resolution, crystals are grown at room temperatur by hanging-drop vapor diffusion, drops contain 0.004 ml HDC at 12.5 mg/ml, 0.001 ml n-dodecyl-beta-D-maltoside and 0.005 ml precipitant solution from the well containing 25% polyethylene glycol 400, 8% polyethylene glycol 400, 100 mM Tris pH 8.0, and 100 mM sodium acetate, enzyme activity is regulated by pH-induced structural changes Lactobacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.22 0.3
-
 L-histidine pH 4.8 Lactobacillus sp.
4.1.1.22 100
-
 L-histidine pH 7.0 Lactobacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.22 L-histidine Lactobacillus sp.
-
 histamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.22 Lactobacillus sp. P00862 30a
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.22
-
 Lactobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.22 L-histidine
-
 Lactobacillus sp. histamine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.22 HDC
-
 Lactobacillus sp.