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Literature summary extracted from

  • Lazard, M.; Agou, F.; Kerjan, P.; Mirande, M.
    The tRNA-dependent activation of arginine by arginyl-tRNA synthetase requires inter-domain communication (2000), J. Mol. Biol., 302, 991-1004.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.19 expression of the native and mutant enzymes in Saccharomyces cerevisiae Cricetulus griseus

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.19 C599Y expression in Saccharomices cerevisiae, shows reduced activity and stability Cricetulus griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.19 additional information
-
 additional information Km values for ATP and L-arginine for several mutants and revertants Cricetulus griseus
6.1.1.19 0.0007
-
 L-arginine ATP-diphosphate exchange reaction, pH 8, 25°C Cricetulus griseus
6.1.1.19 0.0013
-
 L-arginine aminoacylation reaction, pH 8, 25°C Cricetulus griseus
6.1.1.19 0.0023
-
 L-arginine ATP-diphosphate exchange reaction, pH 8, 37°C Cricetulus griseus
6.1.1.19 0.0024
-
 L-arginine aminoacylation reaction, pH 8, 37°C Cricetulus griseus
6.1.1.19 0.0032
-
 tRNAArg aminoacylation reaction, pH 8, 25°C Cricetulus griseus
6.1.1.19 0.0044
-
 tRNAArg aminoacylation reaction, pH 8, 37°C Cricetulus griseus
6.1.1.19 0.19
-
 ATP aminoacylation reaction, pH 8, 25°C Cricetulus griseus
6.1.1.19 0.24
-
 ATP aminoacylation reaction, pH 8, 37°C Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.19 Cricetulus griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.19 purification of the native and mutant enzymes expressed in Saccharomices cerevisiae, using Q-Sepharose and S-Sepharose chromatography Cricetulus griseus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.19 additional information
-
 activity for different mutants Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.19 ATP + L-arginine + tRNAArg
-
 Cricetulus griseus AMP + diphosphate + L-arginyl-tRNAArg
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.19 37
-
 stable for 6 h Cricetulus griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.19 additional information
-
 additional information values for ATP and L-arginine for several mutants and revertants Cricetulus griseus