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Literature summary extracted from

  • Sherlin, L.D.; Bullock, T.L.; Newberry, K.J.; Lipman, R.S.; Hou, Y.M.; Beijer, B.; Sproat, B.S.; Perona, J.J.
    Influence of transfer RNA tertiary structure on aminoacylation efficiency by glutaminyl and cysteinyl-tRNA synthetases (2000), J. Mol. Biol., 299, 431-446.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.16 overexpression in strain JM109 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.16 additional information
-
 additional information tRNAGln mutant substrates Escherichia coli
6.1.1.16 0.0015
-
 tRNACys pH 7.0, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.16 ATP + L-cysteine + tRNACys Escherichia coli
-
 AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.16 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.16 recombinant from overexpressing strain JM109 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.16 ATP + L-cysteine + tRNACys
-
 Escherichia coli AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys mutant tRNA substrate is a tRNACys with mutation at the core tertiary Levitt pair from wild-type G15.C48 to mutant G15.G48, leading to a higher activity Escherichia coli AMP + diphosphate + L-cysteinyl-tRNACys mutant
-
 ?
6.1.1.16 ATP + L-cysteine + tRNAGln duoble-mutant tRNA substrate is a tRNAGln with introduced tRNACys indentitiy nucleotides at the acceptor and anticodon ends and a core tertiary Levitt pair equivalent to tRNAGln of G15.G48, poor activity Escherichia coli AMP + diphosphate + L-cysteinyl-tRNAGln double-mutant
-
 ?
6.1.1.16 ATP + L-cysteine + tRNAGln mutant tRNA substrate is a tRNAGln with introduced tRNACys indentitiy nucleotides at the acceptor and anticodon ends and a core tertiary Levitt pair equivalent to tRNACys of G15.C48 Escherichia coli AMP + diphosphate + L-cysteinyl-tRNAGln mutant
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.16 CysRS
-
 Escherichia coli
6.1.1.16 Cysteine translase
-
 Escherichia coli
6.1.1.16 Cysteine--tRNA ligase
-
 Escherichia coli
6.1.1.16 Cysteinyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.16 Cysteinyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.16 Cysteinyl-tRNA synthetase
-
 Escherichia coli
6.1.1.16 Synthetase, cysteinyl-transfer ribonucleate
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.16 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.16 additional information
-
 additional information tRNAGln mutant substrates Escherichia coli
6.1.1.16 3 6 tRNACys pH 7.0, 37°C Escherichia coli
6.1.1.16 3.47
-
 tRNACys pH 7.0, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.16 7
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.16 ATP
-
 Escherichia coli