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Literature summary extracted from

  • Botos, I.; Meyer, E.; Swanson, S.M.; Lemaitre, V.; Eeckhout, Y.; Meyer, E.F.
    Structure of recombinant mouse collagenase-3 (MMP-13) (1999), J. Mol. Biol., 292, 837-844.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B4 overexpression in Escherichia coli BL21(DE3) Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.B4 enzyme complexed with a hydroxamate inhibitor, hanging-drop vapour diffusion method, 17 mg/ml protein in 10 mM HEPES, pH 7.5, with ammonium sufate to saturation, 18°C, 60 days, X-ray diffraction structure determination at 2.0 A resolution, and analysis Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B4 hydroxamate inhibitor RS-113456 a diphenyl ether sulfon compound, complexing of the enzyme via His205 and Glu202 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B4 Zn2+ His201, His211, and His 205 are involved in metal ion binding, geometry from crystal structure Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B4 protein + H2O Mus musculus
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B4 Mus musculus P33435 precursor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B4 recombinant from Escherichia coli Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.B4 proteolytic degradation of proteins structure of the catalytic domain Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.B4 chondrocyte hypertrophic, enzyme expression during bone development Mus musculus
-
3.4.24.B4 osteoblast enzyme expression during bone development Mus musculus
-
3.4.24.B4 periosteum enzyme expression during bone development Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B4 aggrecan + H2O
-
 Mus musculus ?
-
 ?
3.4.24.B4 cartilage + H2O
-
 Mus musculus ?
-
 ?
3.4.24.B4 collagen type II + H2O
-
 Mus musculus ?
-
 ?
3.4.24.B4 Fibronectin + H2O
-
 Mus musculus ?
-
 ?
3.4.24.B4 protein + H2O
-
 Mus musculus peptides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B4 M10.013 Merops-ID Mus musculus
3.4.24.B4 matrix metalloproteinase-13
-
 Mus musculus
3.4.24.B4 MMP-13
-
 Mus musculus
3.4.24.B4 MMP13
-
 Mus musculus