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Literature summary extracted from

  • Bullard, J.M.; Cai, Y.C.; Demeler, B.; Spremulli, L.L.
    Expression and characterization of a human mitochondrial phenylalanyl-tRNA synthetase (1999), J. Mol. Biol., 288, 567-577.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.20 mitochondrial isozyme, DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.20 0.018
-
 tRNAPhe aminoacylation reaction, recombinant mitochondrial isozyme, pH 7.5, 37°C Homo sapiens
6.1.1.20 0.033
-
 L-phenylalanine ATP-diphosphate exchange reaction, recombinant mitochondrial isozyme, pH 7.3, 37°C Homo sapiens
6.1.1.20 2.5
-
 ATP ATP-diphosphate exchange reaction, recombinant mitochondrial isozyme, pH 7.3, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.20 mitochondrion mitochondrial isozyme Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.20 Mg2+ optimal at 5-10 mM, required, stimulates 2-4fold the second reaction step, the ester bond formation between the 2'-hydroxyl group of the 3'-terminal adenosine base of the tRNA and the carboxylic acid function of phenylalanine Homo sapiens
6.1.1.20 spermine optimal at 1 mM, stimulates 2-4fold the second reaction step, the ester bond formation between the 2'-hydroxyl group of the 3'-terminal adenosine base of the tRNA and the carboxylic acid function of phenylalanine Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.20 45000
-
 recombinant mitochondrial isozyme, gel filtration Homo sapiens
6.1.1.20 48000
-
 recombinant mitochondrial isozyme, analytical velocity sedimentation centrifugation Homo sapiens
6.1.1.20 49600
-
 recombinant mitochondrial isozyme, amino acid sequence determination Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe Homo sapiens
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Homo sapiens O95363 mitochondrial isozyme, class II enzyme
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.20 ATP + L-phenylalanine + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe two-step reaction, the second reaction step is the ester bond formation between the 2'-hydroxyl group of the 3'-terminal adenosine base of the tRNA and the carboxylic acid function of phenylalanine Homo sapiens
a

Storage Stability

EC Number Storage Stability Organism
6.1.1.20 -70°C, purified recombinant His-tagged mitochondrial isozyme, 50 mM Tris-HCl, pH 7.6, 50 mM KCl, 10 mM MgCl2, 7 mM 2-mercaptoethanol, 10% v/v glycerol, small aliquots, stable for at least 6 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Homo sapiens AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ir
6.1.1.20 ATP + L-phenylalanine + tRNAPhe tRNAPhe substrate from Escherichia coli, two-step reaction, the first step, formation of the aminoacyl-adenylate, is reversible, the second, transfer of the activated amino acid to the tRNA, is not Homo sapiens AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ir
6.1.1.20 additional information the enzyme also performs the ATP-diphosphate exchange reaction Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.20 monomer 1 * 48000, recombinant mitochondrial isozyme, analytical sedimentation centrifugation Homo sapiens
6.1.1.20 More the mitochondrial isozyme is a single polypeptide chain, which bears similarities in structure to the alpha/beta subunit organization of bacterial enzymes Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.1.1.20 CML33
-
 Homo sapiens
6.1.1.20 FRS
-
 Homo sapiens
6.1.1.20 HSPC173
-
 Homo sapiens
6.1.1.20 L-Phenylalanyl-tRNA synthetase
-
 Homo sapiens
6.1.1.20 mtPheRS
-
 Homo sapiens
6.1.1.20 Phenylalanine translase
-
 Homo sapiens
6.1.1.20 Phenylalanine--tRNA ligase
-
 Homo sapiens
6.1.1.20 Phenylalanine-tRNA synthetase
-
 Homo sapiens
6.1.1.20 Phenylalanyl transfer ribonucleic acid synthetase
-
 Homo sapiens
6.1.1.20 Phenylalanyl-transfer ribonucleate synthetase
-
 Homo sapiens
6.1.1.20 Phenylalanyl-transfer RNA ligase
-
 Homo sapiens
6.1.1.20 Phenylalanyl-transfer RNA synthetase
-
 Homo sapiens
6.1.1.20 Phenylalanyl-tRNA ligase
-
 Homo sapiens
6.1.1.20 Phenylalanyl-tRNA synthetase
-
 Homo sapiens
6.1.1.20 PheRS
-
 Homo sapiens
6.1.1.20 Synthetase, phenylalanyl-transfer ribonucleate
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.20 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.20 0.11
-
 tRNAPhe aminoacylation reaction, recombinant mitochondrial isozyme, pH 7.5, 37°C Homo sapiens
6.1.1.20 1.1
-
 L-phenylalanine ATP-diphosphate exchange reaction, recombinant mitochondrial isozyme, pH 7.3, 37°C Homo sapiens
6.1.1.20 2.9
-
 ATP ATP-diphosphate exchange reaction, mitochondrial isozyme, pH 7.3, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.20 7.3
-
 ATP-diphosphate exchnage assay at Homo sapiens
6.1.1.20 7.5
-
 aminoacylation assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.20 ATP mitochondrial isozyme, high concentration is required for maximal activity Homo sapiens