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Literature summary extracted from

  • Greenblatt, H.M.; Almog, O.; Maras, B.; Spungin-Bialik, A.; Barra, D.; Blumberg, S.; Shoham, G.
    Streptomyces griseus aminopeptidase: X-ray crystallographic structure at 1.75 A resolution (1997), J. Mol. Biol., 265, 620-636.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.24 protein with or without bound Zn2+ or replaced with Hg2+, hanging drop vapour diffusion method, 20 mg/ml purified enzyme in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM CaCl2, plus an equal volume of sodium acetate buffer at pH 5.0 to 6.0, 16-20% w/v polyethylene glycol 4000, suspended over 1 ml reservoir solution of sodium acetate, pH 5.0-6.0, 16-20% PEG 4000, 4-5 weeks to full size crystals, X-ray diffraction structure determination at 2.1 to 1.75 A resolution and analysis Streptomyces griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.24 Ca2+ location of binding site is in about 25 A distance from the zinc binding site, determination of structural environment Streptomyces griseus
3.4.11.24 Zn2+ 2 mol zinc per mol of enzyme, tightly bound at the active site in a distance of 3.6 A of each other, determination of structural environment Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.24 peptide + H2O Streptomyces griseus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.24 Streptomyces griseus
-
 purified enzyme from commercial product
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.24 release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues M161 is involved in substrate binding at the active site cleft Streptomyces griseus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.24 commercial product protease type XIV i.e. pronase E, a protease mixture form Streptomyces griseus Streptomyces griseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.24 peptide + H2O
-
 Streptomyces griseus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.24 M28.003 Merops-ID Streptomyces griseus
3.4.11.24 SGAP
-
 Streptomyces griseus