BRENDA - Enzyme Database

Production and characterization of extracellular phospholipase D from Streptomyces sp. YU100

Lim, S.K.; Choi, J.W.; Chung, M.H.; Lee, E.T.; Khang, Y.H.; Kim, S.D.; Nam, D.H.; J. Microbiol. Biotechnol. 12, 189-195 (2002)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
Triton X-100
optimal at 1.5%
Streptomyces sp.
General Stability
EC Number
General Stability
Organism
3.1.4.4
relatively stable at high temperature and neutral pH-values, but significantly unstable in the alkaline range
Streptomyces sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
additional information
no inhibitory effect of metal ions
Streptomyces sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
extracellular
secretion during early stage of growth
Streptomyces sp.
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
57000
-
x * 57000, SDS-PAGE
Streptomyces sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Streptomyces sp.
-
YU100, optimal culture conditions
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
-
Streptomyces sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + H2O
hydrolytic and transphosphatylation activity
652769
Streptomyces sp.
1,2-diacylglycerophosphate + choline
-
652769
Streptomyces sp.
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.4
?
x * 57000, SDS-PAGE
Streptomyces sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.4
additional information
-
up to 60°C, increase of activity with increase of temperature
Streptomyces sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
4
-
transphosphatidylation reaction
Streptomyces sp.
3.1.4.4
7
-
hydrolytic reaction
Streptomyces sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
Triton X-100
optimal at 1.5%
Streptomyces sp.
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.4
relatively stable at high temperature and neutral pH-values, but significantly unstable in the alkaline range
Streptomyces sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
additional information
no inhibitory effect of metal ions
Streptomyces sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
extracellular
secretion during early stage of growth
Streptomyces sp.
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
57000
-
x * 57000, SDS-PAGE
Streptomyces sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
-
Streptomyces sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + H2O
hydrolytic and transphosphatylation activity
652769
Streptomyces sp.
1,2-diacylglycerophosphate + choline
-
652769
Streptomyces sp.
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.4
?
x * 57000, SDS-PAGE
Streptomyces sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.4
additional information
-
up to 60°C, increase of activity with increase of temperature
Streptomyces sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
4
-
transphosphatidylation reaction
Streptomyces sp.
3.1.4.4
7
-
hydrolytic reaction
Streptomyces sp.