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Literature summary extracted from

  • Tsiftsoglou, S.A.; Sim, R.B.
    Human complement factor I does not require cofactors for cleavage of synthetic substrates (2004), J. Immunol., 173, 367-375.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.45 antipain 0.1 mM, 80% inhibition of amidolytic activity Homo sapiens
3.4.21.45 Aprotinin 0.0005 mM, 23% inhibition of proteolytic activity. 0.0005 mM, 37% inhibition of amidolytic activity Homo sapiens
3.4.21.45 benzamidine 20 mM, 82% inhibition of amidolytic activity Homo sapiens
3.4.21.45 benzenesulfonyl fluorides inhibits amidolytic activity Homo sapiens
3.4.21.45 benzyloxycarbonyl-D-Phe-Pro-methoxypropylboroglycinepinanediol ester 0.05 mM, 82% inhibition; inhibits amidolytic activity Homo sapiens
3.4.21.45 Cr2+ inhibition of proteolytic and amidolytic activity, 54% inhibition of amidolytic activity at 1 mM, 43% inhibition of proteolytic activity at 0.1 mM Homo sapiens
3.4.21.45 Cu2+ 23% inhibition of amidolytic activity at 1 mM Homo sapiens
3.4.21.45 epsilon-aminocaproic acid 20 mM, 10% inhibition of amidolytic activity Homo sapiens
3.4.21.45 Fe3+ inhibition of proteolytic and amidolytic activity, 59% inhibition of amidolytic activity at 1 mM, 23% inhibition of proteolytic activity at 0.1 mM Homo sapiens
3.4.21.45 Hg2+ 26% inhibition of amidolytic activity at 1 mM Homo sapiens
3.4.21.45 Hirudin
-
 Homo sapiens
3.4.21.45 leupeptin 0.01 mM, 24% inhibition of amidolytic activity; 0.01 mM, 29% inhibition of proteolytic activity Homo sapiens
3.4.21.45 Lima bean trypsin inhibitor 0.05 mM, 20% inhibition of amidolytic activity Homo sapiens
3.4.21.45 additional information no inhibition by 0.1 mM 1,10-phenanthroline, 0.001 mM pepstatin A, 0.1 mM chymostatin, 0.0002 mM, C1 inhibitor and 0.1 mM bestatin Homo sapiens
3.4.21.45 Pefabloc TH 0.25 mM, 58% inhibition of amidolytic activity Homo sapiens
3.4.21.45 Pefabloc-SC 0.00025 mM, complete inhibition of amidolytic activity Homo sapiens
3.4.21.45 Pefabloc-Xa 0.00025 mM, 93% inhibition of amidolytic activity Homo sapiens
3.4.21.45 PMSF 1 mM, 42% inhibition of amidolytic activity Homo sapiens
3.4.21.45 Soybean trypsin inhibitor 0.05 mM, 37% inhibition of amidolytic activity Homo sapiens
3.4.21.45 suramin 1 mM, 90% inhibition of proteolytic activity. 1 mM, 87% inhibition of amidolytic activity Homo sapiens
3.4.21.45 Zn2+ 0.1 mM, 59% inhibition of proteolytic activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.45 0.0146
-
 tert-butyloxycarbonyl-Asp(benzyl ester)-Pro-Arg-4-methylcoumaryl-7-amide pH 8.3, 37°C Homo sapiens
3.4.21.45 0.027
-
 N-alpha-tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide pH 8.3, 37°C Homo sapiens
3.4.21.45 0.128
-
 methylsulfonyl-D-Phe-Gly-Arg-4-methylcoumaryl-7-amide pH 8.3, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.45 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.45 benzoyl-Arg-4-methylcoumaryl-7-amide + H2O low activity Homo sapiens benzoyl-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumaryl-7-amide + H2O low activity Homo sapiens benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens benzyloxycarbonyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 benzyloxycarbonyl-Leu-Leu-Arg-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens benzyloxycarbonyl-Leu-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide + H2O low activity Homo sapiens benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 methylsulfonyl-D-Phe-Gly-Arg-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens methylsulfonyl-D-Phe-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 additional information no cleavage of H-Gly-Arg-7-amido-4-methylcoumarin, MeOSuc-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin or N-alpha-tert-butyloxycarbonyl-leu-Gly-Arg-7-amido-4-methylcoumarin Homo sapiens ?
-
 ?
3.4.21.45 N-alpha-tert-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide + H2O low activity Homo sapiens N-alpha-tert-butyloxycarbonyl-Val-Pro-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 N-alpha-tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens N-alpha-tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 Phe-Gly-Arg-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens Phe-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 Pro-Phe-Arg-4-methylcoumaryl-7-amide + H2O low activity Homo sapiens Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 tert-butyloxycarbonyl-Asp(benzyl ester)-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens tert-butyloxycarbonyl-Asp(benzyl ester)-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 tert-butyloxycarbonyl-Ile-Glu-Gly-Arg-4-methylcoumaryl-7-amide + H2O low activity Homo sapiens tert-butyloxycarbonyl-Ile-Glu-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.45 tert-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumaryl-7-amide + H2O low activity Homo sapiens tert-butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.45 fI
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.45 8.3
-
 reaction with Phe-Gly-Arg-4-methylcoumarin-7-amide Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.45 7 10 pH 7.0: about 45% of maximal activity, pH 10.0: about 45% of maximal activity, reaction with Phe-Gly-Arg-4-methylcoumaryl-7-amide Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.21.45 additional information does not require cofactors for cleavage of synthetic substrates Homo sapiens