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Literature summary extracted from

  • Ambrus, G.; Gal, P.; Kojima, M.; Szilagyi, K.; Balczer, J.; Antal, J.; Graf, L.; Laich, A.; Moffatt, B.E.; Schwaeble, W.; Sim, R.B.; Zavodszky, P.
    Natural substrates and inhibitors of mannan-binding lectin-associated serine protease-1 and -2: a study on recombinant catalytic fragments (2003), J. Immunol., 170, 1374-1382.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B7 catalytic region of MASP-1 and MASP-2, consisting of the two CCP modules and the SP domain (CCP1-CCP2-SP) is expressed in Escherichia coli BL-21 Homo sapiens
3.4.21.104 MASP-2 and truncated fragments of the catalytic region Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B7 alpha2-Macroglobulin may be a physiological inhibitor, 1-3fold molar excess, forms a stable complex with the enzyme Homo sapiens
3.4.21.B7 C1-inhibitor completely blocks enzyme activity, 1-3fold molar excess, forms a stable complex with the enzyme Homo sapiens
3.4.21.104 alpha-2-Macroglobulin forms a stable complex with the enzyme, inhibits at a large molar excess, 40fold Homo sapiens
3.4.21.104 C1-inhibitor forms a stable complex with the enzyme, 1-3fold excess completely inactivates Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B7 0.0048
-
Complement component C2 37°C, cleavage by the MASP-1 CCP1-CCP2-SP fragment Homo sapiens
3.4.21.104 1.6
-
Complement component C4 37°C, pH 7.4 Homo sapiens
3.4.21.104 4
-
Complement component C2 37°C, pH 7.4 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.104 additional information Homo sapiens complement C3 is not a natural substrate ?
-
?
3.4.21.104 Protein + H2O Homo sapiens
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B7 Homo sapiens P48740
-
-
3.4.21.104 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.104
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B7 complement component C2 + H2O digested by the MASP-1 CCP1-CCP2-SP fragment at a moderate rate Homo sapiens ?
-
?
3.4.21.B7 complement component C3i + H2O cleavage of intact C3i (C3 with a reacted thiolester bond) by the MASP-1 CCP1-CCP2-SP fragment with a low but significant efficiency Homo sapiens ?
-
?
3.4.21.B7 complement-activating component of Ra-reactive factor + H2O cleavage at Arg448-/-Ile449 Homo sapiens ?
-
?
3.4.21.B7 MASP1 mannan-binding lectin serine protease 1 isoform 1 precursor + H2O autodegradation pattern of the MASP-1 CCP1-CCP2-SP fragment. Cleavage occurs at the Arg504-Asp505 bond, which results in the removal of a 6000 Da fragment from the active enzyme. The autolysis of the MASP-1 CCP1-CCP2-SP fragment causes the loss of its enzymatic activity due to the removal of the histidine from the catalytic triad Homo sapiens ?
-
?
3.4.21.B7 additional information complement component C4, similar to C3, is basically resistant to the proteolytic activity of MASP-1. MASP-1 shows extreme Arg selectivity Homo sapiens ?
-
?
3.4.21.104 complement component C2 + H2O SP domain alone can autoactivate and cleave C2 Homo sapiens ?
-
?
3.4.21.104 complement component C3i + H2O
-
Homo sapiens ?
-
?
3.4.21.104 complement component C4 + H2O SP domain is able to cleave C4, but the presence of the CCP2 domain significantly increases the efficiency Homo sapiens ?
-
?
3.4.21.104 additional information hardly any detectable activity on complement C3 Homo sapiens ?
-
?
3.4.21.104 additional information complement C3 is not a natural substrate Homo sapiens ?
-
?
3.4.21.104 Protein + H2O
-
Homo sapiens ?
-
?
3.4.21.104 Protein + H2O cleaves oligopeptides that contain either Arg or Lys at their P1 positions Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B7 mannan-binding lectin-associated serine protease-1
-
Homo sapiens
3.4.21.104 MASP-2
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B7 0.1
-
Complement component C2 37°C, cleavage by the MASP-1 CCP1-CCP2-SP fragment Homo sapiens
3.4.21.104 0.9
-
Complement component C4 37°C, pH 7.4 Homo sapiens
3.4.21.104 1.9
-
Complement component C2 37°C, pH 7.4 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.104 0.0000088
-
C1-inhibitor 37°C, pH 7.4 Homo sapiens