EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.21.B7 | catalytic region of MASP-1 and MASP-2, consisting of the two CCP modules and the SP domain (CCP1-CCP2-SP) is expressed in Escherichia coli BL-21 | Homo sapiens |
3.4.21.104 | MASP-2 and truncated fragments of the catalytic region | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.B7 | alpha2-Macroglobulin | may be a physiological inhibitor, 1-3fold molar excess, forms a stable complex with the enzyme | Homo sapiens | |
3.4.21.B7 | C1-inhibitor | completely blocks enzyme activity, 1-3fold molar excess, forms a stable complex with the enzyme | Homo sapiens | |
3.4.21.104 | alpha-2-Macroglobulin | forms a stable complex with the enzyme, inhibits at a large molar excess, 40fold | Homo sapiens | |
3.4.21.104 | C1-inhibitor | forms a stable complex with the enzyme, 1-3fold excess completely inactivates | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.B7 | 0.0048 | - |
Complement component C2 | 37°C, cleavage by the MASP-1 CCP1-CCP2-SP fragment | Homo sapiens | |
3.4.21.104 | 1.6 | - |
Complement component C4 | 37°C, pH 7.4 | Homo sapiens | |
3.4.21.104 | 4 | - |
Complement component C2 | 37°C, pH 7.4 | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.104 | additional information | Homo sapiens | complement C3 is not a natural substrate | ? | - |
? | |
3.4.21.104 | Protein + H2O | Homo sapiens | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.B7 | Homo sapiens | P48740 | - |
- |
3.4.21.104 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.104 | - |
Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.B7 | complement component C2 + H2O | digested by the MASP-1 CCP1-CCP2-SP fragment at a moderate rate | Homo sapiens | ? | - |
? | |
3.4.21.B7 | complement component C3i + H2O | cleavage of intact C3i (C3 with a reacted thiolester bond) by the MASP-1 CCP1-CCP2-SP fragment with a low but significant efficiency | Homo sapiens | ? | - |
? | |
3.4.21.B7 | complement-activating component of Ra-reactive factor + H2O | cleavage at Arg448-/-Ile449 | Homo sapiens | ? | - |
? | |
3.4.21.B7 | MASP1 mannan-binding lectin serine protease 1 isoform 1 precursor + H2O | autodegradation pattern of the MASP-1 CCP1-CCP2-SP fragment. Cleavage occurs at the Arg504-Asp505 bond, which results in the removal of a 6000 Da fragment from the active enzyme. The autolysis of the MASP-1 CCP1-CCP2-SP fragment causes the loss of its enzymatic activity due to the removal of the histidine from the catalytic triad | Homo sapiens | ? | - |
? | |
3.4.21.B7 | additional information | complement component C4, similar to C3, is basically resistant to the proteolytic activity of MASP-1. MASP-1 shows extreme Arg selectivity | Homo sapiens | ? | - |
? | |
3.4.21.104 | complement component C2 + H2O | SP domain alone can autoactivate and cleave C2 | Homo sapiens | ? | - |
? | |
3.4.21.104 | complement component C3i + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.104 | complement component C4 + H2O | SP domain is able to cleave C4, but the presence of the CCP2 domain significantly increases the efficiency | Homo sapiens | ? | - |
? | |
3.4.21.104 | additional information | hardly any detectable activity on complement C3 | Homo sapiens | ? | - |
? | |
3.4.21.104 | additional information | complement C3 is not a natural substrate | Homo sapiens | ? | - |
? | |
3.4.21.104 | Protein + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.104 | Protein + H2O | cleaves oligopeptides that contain either Arg or Lys at their P1 positions | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.B7 | mannan-binding lectin-associated serine protease-1 | - |
Homo sapiens |
3.4.21.104 | MASP-2 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.B7 | 0.1 | - |
Complement component C2 | 37°C, cleavage by the MASP-1 CCP1-CCP2-SP fragment | Homo sapiens | |
3.4.21.104 | 0.9 | - |
Complement component C4 | 37°C, pH 7.4 | Homo sapiens | |
3.4.21.104 | 1.9 | - |
Complement component C2 | 37°C, pH 7.4 | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.104 | 0.0000088 | - |
C1-inhibitor | 37°C, pH 7.4 | Homo sapiens |