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Literature summary extracted from

  • Coursol, S.; Pierre, J.N.; Vidal, J.; Grisvard, J.
    Cloning and characterization of a phospholipase C from the C4 plant Digitaria sanguinalis (2002), J. Exp. Bot., 53, 1521-1524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.11 full-length DsPLC2 cDNA, expression of two proteins synthesized from two potential initiation codons in Escherichia coli BL21, sequencing Digitaria sanguinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.11 additional information not inhibited by U-73343 Digitaria sanguinalis
3.1.4.11 U-73122 aminosteroid inhibitor¸ the two recombinant enzymes synthesized from two potential initiation codons differ in the sensitivity Digitaria sanguinalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.11 cytosol DsPLC2 is most likely located in the cytosol Digitaria sanguinalis 5829
-
3.1.4.11 protoplast DsPLC2, mesophyll cell protoplasts from leaves Digitaria sanguinalis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.11 Ca2+ activates, the two recombinant enzymes synthesized from two potential initiation codons differ in their Ca2+ requirements Digitaria sanguinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.11 70800
-
 x * 70800, DsPLC2, calculated from the amino acid sequence Digitaria sanguinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.11 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O Digitaria sanguinalis plays a role in various signal transduction pathways, DsPLC2 plays a role in an early event in the light induction process of C4 photosynthesis 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol the products act as second messengers in cell signalling ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.11 Digitaria sanguinalis Q9FSW1 DsPLC2, 6 weeks old
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.11 recombinant DsPLC2 Digitaria sanguinalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.11 leaf DsPLC2, from 6 weeks old plants Digitaria sanguinalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.11 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Digitaria sanguinalis 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?
3.1.4.11 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O plays a role in various signal transduction pathways, DsPLC2 plays a role in an early event in the light induction process of C4 photosynthesis Digitaria sanguinalis 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol the products act as second messengers in cell signalling ?

Subunits

EC Number Subunits Comment Organism
3.1.4.11 ? x * 70800, DsPLC2, calculated from the amino acid sequence Digitaria sanguinalis

Synonyms

EC Number Synonyms Comment Organism
3.1.4.11 DsPLC2
-
 Digitaria sanguinalis