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Literature summary extracted from

  • Okorukwu, O.; Vercruysse, K.P.
    Effects of ascorbic acid and analogs on the activity of testicular hyaluronidase and hyaluronan lyase on hyaluronan (2003), J. Enzyme Inhib. Med. Chem., 18, 377-382.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.35 D-isoascorbic acid
-
 Bos taurus
3.2.1.35 dehydroascorbic acid weak inhibition Bos taurus
3.2.1.35 L-ascorbic acid
-
 Bos taurus
3.2.1.35 additional information no inhibition by saccharic acid, poor inhibitors are alpha-D-glucoheptonic-gamma-lactone, D-saccharic-1,4-lactone, L-gulonic-gamma-lactone, D-ribonic-gamma-lactone, and D-gluconic-gamma-lactone Bos taurus
4.2.2.1 D-isoascorbic acid strong inhibition Streptomyces hyalurolyticus
4.2.2.1 D-saccharic-1,4-lactone strong inhibition Streptomyces hyalurolyticus
4.2.2.1 dehydroascorbic acid
-
 Streptomyces hyalurolyticus
4.2.2.1 L-ascorbic acid strong inhibition Streptomyces hyalurolyticus
4.2.2.1 additional information poor inhibitors are alpha-D-glucoheptonic-gamma-lactone, L-gulonic-gamma-lactone, D-ribonic-gamma-lactone, and D-gluconic-gamma-lactone Streptomyces hyalurolyticus
4.2.2.1 saccharic acid
-
 Streptomyces hyalurolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.35 NaCl
-
 Bos taurus
4.2.2.1 NaCl
-
 Streptomyces hyalurolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Bos taurus
-
-
-
4.2.2.1 Streptomyces hyalurolyticus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.35 hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide hydrolase mechanism Bos taurus
a
4.2.2.1 [hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate lyase mechanism, elimination process Streptomyces hyalurolyticus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 commercial preparation testicular enzyme Bos taurus
-
3.2.1.35 testicle
-
 Bos taurus
-
4.2.2.1 commercial preparation
-
 Streptomyces hyalurolyticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronan + 2 H2O from Streptococcus zooepidemicus, degradation Bos taurus N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?
3.2.1.35 additional information hyaluronan is degraded by L-ascorbic acid, D-isoascorbic acid, and dehydroascorbic acid themselves, only slightly by L-gulonic-gamma-lactone, D-saccharic-1,4-lactone, and alpha-D-glucoheptonic-gamma-lactone Bos taurus ?
-
 ?
4.2.2.1 hyaluronan + 2 H2O from Streptococcus zooepidemicus, degradation Streptomyces hyalurolyticus N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?
4.2.2.1 additional information hyaluronan is degraded by L-ascorbic acid, D-isoascorbic acid, and dehydroascorbic acid themselves, only slightly by L-gulonic-gamma-lactone, D-saccharic-1,4-lactone, and alpha-D-glucoheptonic-gamma-lactone Streptomyces hyalurolyticus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 testicular HAse
-
 Bos taurus
3.2.1.35 testicular hyaluronidase
-
 Bos taurus
4.2.2.1 bacterial HAse
-
 Streptomyces hyalurolyticus
4.2.2.1 bacterial hyaluronidase
-
 Streptomyces hyalurolyticus
4.2.2.1 hyaluronan lyase
-
 Streptomyces hyalurolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 22
-
 assay at room temperature Bos taurus
4.2.2.1 22
-
 assay at room temperature Streptomyces hyalurolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 6.5
-
 assay at Bos taurus
4.2.2.1 6.5
-
 assay at Streptomyces hyalurolyticus